【作者】 孟宪丽； 熊玉霞； 杨娜； 张艺； 何毓敏；

【Author】 Xiong Yuxia Meng Xianli Yang Na (School of Pharmacy of Chengdu University of TCM,Chengdu 610075)

【机构】 成都中医药大学药理教研室；

【摘要】 目的：观察泻心汤有效组分结合蒽醌与总黄酮配伍(重量比为4：3)对 LPS 诱导巨噬细胞 NO 合成以及对 iNOS、 CD14和 TLR4mRNA 表达的影响。方法：采用 MTT 法观察结合蒽醌与总黄酮配伍对巨噬细胞生长活性的影响,采用 Griess 法观察其对 LPS 诱导的巨噬细胞产生 NO 的影响,采用 RT-PCR 法观察其对巨噬细胞 iNOS、CD14和 TLR4mRNA 表达在2、4、6、24h 的影响。结果：泻心汤结合蒽醌与总黄酮配伍在0．01～0．1 mg/ml 剂量对巨噬细胞生长活性无明显影响,此浓度能明显抑制 LPS 诱导的巨噬细胞 NO 分泌,结合蒽醌与总黄酮配伍组 TLR4和 CD14mRNA 表达在2h 明显低于 LPS 模型组,而 iNOSmRNA 表达与模型组无明显差异,4h 以后 iNOS 和 TLR4mRNA 的表达均明显低于模型组,而 CD14mRNA 表达在4h 后与模型组无明显差异。结论：泻心汤结合蒽醌与总黄酮配伍抑制 LPS 诱导的巨噬细胞 NO 合成、iNOSmRNA 表达与其抑制 TLR4mRNA 的表达密切相关,而与 CD14的关系不大,TLR4可能是泻心汤结合蒽醌与总黄酮配伍抗内毒素的作用靶点。更多还原

【Abstract】 Objective:To observe the effect of compatibility of Conjunct anthraquinone and total flavonoids (proportion by weight is 4:3 ) on NO production,and expression of iNOS,CDl4 and TLR4mRNA in peritoneal macrophages induced by LPS.Methods:Growth of macrophages cultured with different levels of the compatibility was determined by MTT,NO production in macrophages induced by LPS was detected by Griess method,the expression of iNOS,CDl4 and TLR4mRNA in macrophages at the time of 2,4,6 and 24h induced by LPS were detected by RT-PCR method.Results:Therewas no obvious influence on growth activity of macrophages at dose of 0.01～0.I mg/ml of the com- patibility,the NO level was inhibited obviously by the compatibility at those dose after 4h,the expression of TLR4 and CD14mRNA were in- hibited obviously by the compatibility compared with LPS group at 2h (P＜0.05),but the expression of iNOSmRNA had no obvious difference between the compatibility and LPS group at the same time,the expression of TLR4 and iNOSmRNA were inhibited obviously after 4h(P＜0. 05),but the expression of CDl4mRNA showed no significant difference between the compatibility and LPS group at the same time after 4h (P ＜0.05).Conclusion:The results suggested that the down-regulation of NO production and expression of iNOS mRNA were related with TLR4,but less with CDl4,TLR4 was the possible antiendotoxic target of the compatibility of active components of Xiexin Decoction.更多还原