【作者】 刘爽； 朴丰源； 姚晓峰； 曲淑贤； 李秋娟； 孙鲜策； 叶建新；

【Author】 LIU Shuang~1 PIAO Feng-yuan~1 YAO Xiao-feng~1 QU Shu-xian~2 LI Qiu-juan~1 SUN Xian-ce~1 YE Jian-xin~1 ( 1.Department of Hygiene,College of Basic Medical Sciences,Dalian Medical University,Dalian Liaoning 116027,China)

【机构】 大连医科大学卫生学教研室； 大连医科大学中心实验室；

【摘要】 目的探讨砷对肾组织 DNA 氧化性损伤以及牛磺酸和维生素 C 的保护作用。方法小鼠60只,随机分为6组,即：低剂量组（1 mg/L As₂O₃）、中剂量组（2 mg/L As₂O₃）、高剂量组（4 mg/L As₂O₃）、牛磺酸保护组（4 mg/L As₂O₃+ 150 mg/kg牛磺酸）、维生素 C 保护组（4 mg/L As₂O₃+45 mg/kg 维生素 C）以及生理盐水对照组,用 HE 染色法进行组织病理学观察,用免疫组化检测肾组织内8-OH-dG 的水平。结果各染砷组小鼠肾组织出现不同程度细胞肿胀．胞浆空泡变性等病理学改变。免疫组化表明砷暴露组8-OH-dG 的表达显著增高。牛磺酸和维生素 C 保护组肾组织病理学变化较轻,8-OH-dG 表达较少（P＜0．01）。结论慢性砷暴露可引起小鼠肾组织 DNA 氧化性损伤和病理变化,牛磺酸和维生素 C 具有保护作用。更多还原

【Abstract】 Objective To evaluate the protective effects of taurine and vitamin C on arsenium（As）exposed mice kidney. Methods 60 mice were divided into 6 groups,low dose group（1mg/L Arsenic trioxide（As₂O₃））,medium dose group（2 mg/L As₂O₃）,high dose group（4 mg/L As₂O₃）,taurine protective group（4 mg/L As₂O₃ + 150mg/kg taurine）,vitamin C protective group （4mg/L As₂O₃ + 45mg/kg vitamin C）,and normal saline group as control.Kidney tissues were taken and performed with HE staining and 8 hydroxy-2′-deoxyguanine expression were examined by immunohistochemistry.Results The kidney cells in As treated groups showed swelling and vacuolation.8-OH-dG were also strongly expressed in As exposed groups.Two prophylactic groups showed very mild damages with very low 8-OH-dG level（P＜0.01）.Conclusions The kidneys showed severe pathologic injuries and DNA oxidative damages after long-term As exposure.Tanrine and vitamin C alleviated the damage significantly.更多还原