【作者】 王维亭； 赵专友； 刘厚孝；

【Author】 WANG Wei-Ting, ZHAO Zhuan-You, LIU Hou-Xiao(Center far the New Drugs Evaluation ,Tianjin Institute of Pharmaceutical Research, Tianjin 300193)

【机构】 天津药物研究院新药评价中心；

【摘要】 目的:研究不稳定型心绞痛模型建立方法,并观察肝素钠的疗效。方法:采用犬冠脉左旋支的机械损伤与狭窄,造成了冠脉流量的周期性降低(CFRs),建立了不稳定型心绞痛模型,并观察了肝素钠的疗效。结果:结果表明,犬冠脉内膜经机械损伤及冠脉狭窄后,引起的血小板依赖性血栓形成,出现冠脉流量的周期性降低(CFRs),且3h内CFRs基本保持稳定,提示模型稳定性可靠;肝素钠0.75、1.5、3.0 mg/kg可使周期性冠脉流量下降发生次数减少,抗凝血参数TT、PT、APTT不同程度延长,同时肝素钠可使麻醉犬粘膜出血时间延长及伤口失血量增多。结论:研究结果表明,该模型稳定可靠;肝素钠对不稳定型心绞痛疗效显著,其疗效与肝素抗凝、抗栓作用有关。更多还原

【Abstract】 AIM: This research established the model of unstable angina pectoris in mongrel dogs, and observe the therapeutic effects of heparin on angina pectoris. METHODS: Cyclic coronary flow reductions were induced in the left circuflex coronary artery of 30 anaesthetised mongrel dogs by mechanical injury of the endothelium combined with critical stenosis. RESULT: After mechanical injury and critical stenosis , the platelet- dependent thrombus was formatted in coronary artery , the frequency of CFRs kept stability in 3 hours. A linear decrease in CFRs to 7. 8±2. 4 (Cycle per hour,cph ) , 6. 2±1. 7cph, 4. 5± 0. 8cph was achieved with the three doses of heparin. The thrombin time (TT), the partial thromboplastin time(APTT) was increased significantly. Buccal bleeding time and blood loss were increased after either treatment . CONCLUSION: The results suggested that the model of unstable angina pectoris is realizable , and heparin has a therapeutic effects by antithrombotic and anticoagulatting characteristics.更多还原