【作者】 阚光锋； 张广民； 房保海； 迟长凤；

【Author】 Kan Guang-feng, Zhang Guang-min, Fang Bao-hai, Chi Chang-feng College of plant protection, Shandong Agricultural University, Taian 271018

【机构】 山东农业大学植保学院植物病理系；

【摘要】 烟草叶片接种烟草野火病菌的酶活性测定结果表明,SOD在各烟草品种中含量大致相同,接种后活性只略有升高,同工酶电泳图谱中只产生两条酶带;PAL和CAT含量在接种野火病菌前无规律性,但接种后在抗性品种中降低而在感性品种中升高;接种前POD的含量与品种抗性呈正相关,而PPO含量与品种抗性呈负相关,可作为烟草对野火病抗性的生理生化指标。同工酶谱带中,接种后POD比接种前多了1~2条酶带,而PPO少了1~2条酶带。更多还原

【Abstract】 By the determinationon some enzymes activites of tabacco leaves inoculated with Pseudomonas syringae pv. tabaci(p. s. pv. tabaci), the resulsts showed that every enzyme changed differently. The activity of SOD increased slightly after challenge inoculation in four tabacco varieties. There were only two bands in zymogram before and after inoculating. The actevities of PAL and CAT were irrigular before inoculation., while decreased in resistant rarities and increased in susceptible ones after inoculation. There was a negative correlation of the activity of POD with the level of p. s. pv. tabaci infection. On the contrary, there was a positive correlation bewteen the level of p. s. pv. tabaci infection and the activity of PPO. Tabacco leaves inoculated with p. s. pv. tabaci, showed higher isoenzymatic activities and exposed one or two more bands in POD zymogram as compared with control leaves, while showed lower isoenzymatic activities and exposed one or two less bands in PPO zymogra.更多还原