【作者】 敖登高娃； 斯琴； 赵永亮；

【Author】 Ao Deng-gaowa SI-QIN ZHAO-Yong Liang (College of Chemistry and Chemical Engineering Neimongol University,Hohht,010021)

【机构】 内蒙古大学化学化工学院；

【摘要】 在 pH7.0±0.1的 Tris-HCl 缓冲溶液中,Eu³⁺与加替沙星形成的配合物受270nm 紫外光激发发出 Eu³⁺的特征荧光峰,加入脱氧核糖核酸（DNA）能大大增强体系的荧光强度,由此建立 GFLX-Eu³⁺配合物探针测定核酸的方法。与 GFLX-Eu³⁺二元配合物相比, GFLX-Eu³⁺-DNA 三元体系荧光强度显著增强,且最大吸收波长紫移5nm,表明形成三元配合物。研究了反应的最佳条件,并初步探讨了 GFLX-Eu³⁺配合物探针和 DNA 结合机理。更多还原

【Abstract】 The experiments indicated that europium（Ⅲ） ion could complex with gatifloxacin in pH7.0±0.1 Tris-HCl buffer solution,then emitted the characteristic fluorescence of europium（Ⅲ） ion.While the DNA was added,the fluorescence intensity of the syetem was greatly increased. Based on this,a sensitive method for the determination of DNA was established.Compared with the binary complex of GFLX-Eu³⁺ the ternary systems of GFLX-Eu³⁺-DNA have larger fluorescence intensity and their maximal absorptions are blue-shifted about 5nm,indicated that an ternary complex was found.The optimum reaction conditions were discussed and the mechanism of the ternary complex was preliminary studied.更多还原