【作者】 李升和； 金光明； 王珏； 顾有方； 彭克美； 刘坤；

【Author】 LI Sheng-he~(1,2) JIN Guang-ming~1 WANG Jue~1 GU You-fang~1 PENG Ke-mei~1 LIU Kun~1 (1.Anhui Science and Technology University,Fengyang 233100; 2 Huazhong Agricultural University,Wuhan 430070)

【机构】 安徽科技学院； 华中农业大学；

【摘要】 目的研究决明子水煎液对CCl₄所致大鼠急性肝损伤的保护作用及其作用机制。方法48只体重180～240g SD大鼠,随机分为正常对照组、模型对照组、决明子Ⅰ组和决明子Ⅱ组,每组12只,正常对照组和模型对照组大鼠饮用蒸馏水,决明子Ⅰ组和决明子Ⅱ组分别饮用浓度为40g/L和80g/L决明子水煎液,连用14d后,模型对照组、决明子Ⅰ组和决明子Ⅱ组腹腔注射CCl₄（2.0ml/kg体重）,正常对照组注射等体积生理盐水,24h后10%水合氯醛麻醉,右心房采血,低速离心,取血清分别测门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-PX）和过氧化氢酶（CAT）水平:同时立即解剖取肝脏,Bouin液固定,制作石蜡切片,HE染色,观察肝脏组织结构变化。结果决明子水煎液能显著降低CCl₄所致的SD大鼠血清中AST、ALT及MDA含量的升高（P<0.01）,SOD、GSH-PX的活性显著升高（P<0.01）,CAT活性有所提高;决明子Ⅰ组和决明子Ⅱ组大鼠肝脏的病理组织学变化较模型对照组明显好转,其中决明子Ⅰ组的保肝效果要比决明子Ⅱ组好。结论决明子水煎液对CCl₄所致的大鼠急性肝损伤有一定保护作用。更多还原

【Abstract】 Objective:Studied the protection of Semen cassiae water extract against the acute injure of liver induced by CCL₄ in rats and probe into its mechanism.Methods:A total of 48 SD rat of 180-240g body weight were randomly divided into normal control group,model control group,experiment groupⅠand experiment groupⅡ,the normal control group and model control group both drank distilled water,and the experiment groupⅠandⅡdrank 40g/l and 80g/l Semen cassiae water extract respectively for two weeks,The model control group,the experiment groupⅠandⅡwere injected in abdominal cavity with CCl₄ （0.2g/kg body weight）,the normal control group was injected with isotonic Na chloride of the same volume.After 24 hours,the rats of each treatment were made into narcosis with 10% Chloral Hydrate,the blood were obtained in right atria,and were centrifugated in low speed,Then the serum were obtained and estimated the level of the AST, ALT,SOD,MDA,GSH-PX and CAT.The livers were obtained at the same time,fixed with Bouin fixation fluid,made into paraffin section and stained with HE,and the microstructure of liver were observed by microscope.Result:Compared with the model control group,the serum AST,ALT and MDA levels of experimentⅠandⅡdecreased significantly （p<0.01）,but the activity of SOD,GSH-PX increased significantly （p<0.01）,and the activity of CAT raised a little.Under the microscope, the pathohistology changes of liver of experiment groupⅠandⅡimproved obviously,and the protection of experiment groupⅠwere better than experiment groupⅡ.Conclusion:The results showed that the semen cassiae water extract could protect the liver of rats which were damaged by CCl₄.更多还原