【作者】 李梅英； 张莉； 包慧君； 黑乃楠； 陈秋生；

【Author】 LI Meiying ZHANG Li BAO Huijun HEI Nai-nang CHEN Qiusheng (College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)

【机构】 南京农业大学动物医学院；

【摘要】 研究雄激素受体(androgen receptor,AR)mRNA在中华鳖(Pelodiseus Sinensis)脾脏的存在及细胞定位。取5只健康成年中华鳖的脾脏,进行石蜡切片。用地高辛标记的寡核苷酸探针对组织切片进行原位杂交(in situ hybridization,ISH),检测雄激素受体(androgen receptor,AR)mRNA在脾脏免疫细胞内的表达定位。原位杂交结果显示,大部分雄激素受体mRNA阳性细胞呈圆形或椭圆形,数量少胞体大且呈不规则形。阳性细胞在脾脏动脉周围淋巴鞘(Periaterial lymphatic sheath,PALS)和红髓(Red pulp,RP)内分布密集。椭球周围淋巴鞘(periellipsoidal lymphatic sheath,PELS)内AR mRNA阳性细胞分布极少。杂交阳性信号物质在细胞质和细胞核均有分布。AR mRNA分布于中华鳖脾脏免疫细胞中,且推测圆形的阳性细胞可能为B淋巴细胞而另一部分胞体大且形状不规则的阳性细胞则可能为巨噬细胞。脾脏内AR mRNA的存在进一步证实雄激素可能是调节脾脏等免疫器官功能的因素之一。更多还原

【Abstract】 The reptile immune system is strikingly affected by seasonal variations,which induce changes in the structure of the lymphoid organs and in the function of the leukocytes.Morphological changes of lymphoid organs (thymus and spleen) throughout the year may relate to annual variations of circulating steroid hormones.Androgen is a kind of steroid hormones and may have direct effects mediated via the androgen receptor.Therefore,to study whether androgen receptor mRNA exists in spleen of Pelodiseus Sinensis and its cellular localization,which is very important.Paraffin sections of spleen in five healthy Pelodiseus Sinensis.In situ hybridization (ISH) was conducted on the tissue sections by oligonucleotide probe marked by cardiox.The expressions and localization of androgen receptor mRNA were detected in the cells of spleen tissue. The result of in situ hybridization indicated that the distribution of AR positive cells in the Periaterial lymphatic sheath (PALS) and red pulp were more than those in the periellipsoidal lymphatic sheath (PELS).The results of this study for the first time demonstrate that AR mRNA indeed exists in Pelodiseus Sinensis immune cells of spleen.Most of AR mRNA positive cells are round or oval-shap,the others are lager and irregular.The signals were distributed in cytoplasm and nuclei of all positive cells.The results suggest that the round or oval-shap cells might be B lymphocyte,the others might be macrophages.The presence of androgen receptor mRNA in the spleen would provide further evidence that androgen might be one of factors which effect on function of spleen and the other immune organs.更多还原