【作者】 温建新； 王金宝； 李俊； 任慧英； 刘文华； 邹玲； 支兵杰； 张利娜；

【Author】 WEN Jianxin, WANG Jinbao ,LI Jun,REN Huiying, LIU Wenhua, ZHOU Lin Zhi Binjie Zhang Lina (Shan Dong Agriculture Univisty, Taian 265200; Agriculture Research institute SAAS, Jinan,250000; Lai Yang Agriculture Univisty ,Qing Dao 266109)

【机构】 山东农业大学动物科技学院； 山东省农业科学院； 莱阳农学院；

【摘要】 为了筛选出对猪体有免疫刺激活性的CpG寡聚脱氧核苷酸(CpG-ODN),设计了9种未甲基化CpG-ODN不同序列,通过人工合成,分别作用于猪外周血淋巴细胞和脾细胞,进行健康猪外周血淋巴细胞和脾脏免疫细胞体外转化试验。用四甲基偶氮唑盐(MTT)比色法检测外周血淋巴细胞转化效果和脾细胞的增殖能力和代谢能力,通过测得的OD490值,计算出淋巴细胞增殖指数(SI),筛选 CpG-ODN的免疫刺激作用。结果显示:九种不同序列的CpG-ODN对猪外周血淋巴细胞和脾脏免疫细胞均具有不同程度的刺激效果,其中CpG-ODN6、CpG-ODN7刺激强度极显著。为研制DNA疫苗有效的CpG-ODN免疫佐剂奠定基础。更多还原

【Abstract】 In the study, nine different artificial unmethylated oligodexynucleotides (CpG-ODN) sequences based on the swine peripheral blood lymph cells and spleen cells respectively. The effect of the invitro lymphocyte transformation was detected by MTT assay. According to the tested OD , calculated the lymph cells SI to study the immune effect of CpG-ODN. It is showed that the nine CpG-ODNs had different extent of immunostimulatory effects on the swine peripheral blood lymph cells. The stimulation index of CpG-ODN was screened out ..After elaborate analysis ,the conclusion was drawn that the CpG-ODN6 had the strongest immunostimulatory activity on the swine peripheral blood lymph cells. This would be useful for study of effective CpG-ODN immune adjunct for gene vaccination in future.更多还原