【作者】 张路培； 许尚忠； 张小辉； 高雪； 任红艳； 陈金宝；

【Author】 ZHANG Lu-pei, XU Shang-zhong,ZHANG Xiao-hui, GAO Xue,REN Hong-yan, CHEN Jin-bao (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100094, China Animal Science-Technology College of Northwest A & F University, Yangling, 712100, China)

【机构】 中国农业科学院畜牧研究所； 西北农林科技大学动物科技学院；

【摘要】 根据缺失突变的特殊性,应用创造酶切位点和PCR产物直接经聚丙烯酰胺凝胶两种方法检测缺失突变的基因型。并以牛生长分化因子9(growth differentiation factor 9,GDF9)基因和骨形态发生蛋白15(bone morphogenetic protein 15,BMP15)基因的缺失突变基因型的检测为例,对两种方法进行了探讨。更多还原

【Abstract】 According to the particularity of deletion, deletion genotype were detected with Created Restriction Site PCR and direct electrophoresis with PCR products. In the study, deletion of growth differentiation factor 9 gene and bone morphogenetic protein 15 gene were served as examples for establishing these methods.更多还原