【作者】 冯海华； 范君文； 赵丽红； 成军； 邓旭明； 岳占碰；

【Author】 FENG Hai-hua, FAN Jun-wen, ZHAO Li-hong, CHENG Jun, DENG Xu-ming, YUE Zhan-peng (College of Animal Science and Veterinary Medicine, Jilin University,Changchun 130062, China)

【机构】 吉林大学畜牧兽医学院；

【摘要】 X型胶原(colX)是肥大软骨细胞的标志物,根据GenBank中收录的人、鼠、牛、猪X型胶原基因的序列,进行同源性分析,在同源性高的相对保守区域设计了1对特异性引物。培养鹿茸软骨细胞,提取细胞的总RNA,以总RNA为模板,用RT-PCR方法克隆了梅花鹿X型胶原基因序列其中的877bp的片段,此片段全部位于编码区,与已报道的牛的X型胶原基因的序列比较,同源性达到96％。共有35个碱基发生变异。DNAStar软件分析表明,二者推导的氨基酸序列同源性为95．5％。更多还原

【Abstract】 The collagen X protein is the marker of hypertrophic chondrocytes. The homology was analyzed based on the reported collagen X sequence of human, mouse, cow and pig in GenBank. A pair of specific primers was designed in the relatively conservative sectors. The Sika deer antler chondrocytes were cultured, total RNA was extracted from the cultured cells. The sequence encoding deer antler collagen X was cloned from the cartilage cells of sika deer antler by means of the specific primers with the methods of RT-PCR. The cloned sequence was 877 bp with an ORF. This sequence encoded 292 amino acids. The homology of the nucleotide sequence of sika deer collagen X with cow was 96%, and the homology of deduced amino acids between them was 95.5%.更多还原