【作者】 陈士恩； 马忠仁； 冯玉萍； 毛东风； 罗晓琴；

【Author】 CHEN Shi-en MA Zhong-ren FENG Yu-píng MAO Dong-feng LUO Xiao-qin (life sciences and engineering college of Northwest Minorities University, Lanzhou Gansu 730030, China)

【机构】 西北民族大学生命科学与工程学院；

【摘要】 成功研制了检测绵羊巴氏杆菌抗体的间接ELISA试剂盒。以提取的巴氏杆菌外膜蛋白和脂多糖为该试剂盒的包被抗原,其最佳工作浓度为稀释200倍,包被浓度为5 μg／ml。所检血清最佳稀释度为1:100。辣根过氧化物酶标兔抗羊IgG最佳工作浓度为1:800。在甘肃省某羊场绵羊巴氏杆菌病爆发期间,利用该间接ELISA方法检测1800份绵羊血清,其中 56份呈阳性,并从15例死羊分离出同型溶血性巴氏杆菌毒株。更多还原

【Abstract】 Used a separation sheep Pasteuralla to develop the ELISA kit to measure its antibody. The diaper antigen is the extraction Pasteuralla outer membrane protein and the lipopolysaccharide, The best work density is diluted 200 times, The diaper density probably is 5 μg/ ml.The measuring blood serums best degree of dilution is 1:100. The best work density about HRP-IgG of rabbit anti-sheep is 1:800. According to conventional indirect Elisa method , can measure sheep blood serum Pasteuralla antibody effectively.更多还原