【作者】 刘菲； 程安春； 汪铭书； 宋涌； 廖永洪； 袁桂萍； 韩晓英； 徐超；

【Author】 LIU Fei, CHENG An-chun, WANG Ming-shu, SONG Yong, LIAO Yong-hong, YUAN Gui-ping, HAN Xiao-ying, XU Chao(Avian Disease Research Center, College of Animal Science & Veterinary Medicine, Sichuan Agricultural University, Yaan, Sichuan,China, 625014)

【机构】 四川农业大学动物科技学院禽病研究中心；

【摘要】 56只3月龄四川麻鸭经皮下接种鸭瘟病毒(DPV)强毒SC1株成功建立了DPV感染的急性病理模型,并应用PCR方法检测不同时间DPV在感染鸭体各组织器官的分布情况。结果表明,接种2小时后即能够从脑、肝、脾、法氏囊、胸腺中检出DPV DNA。12小时,即可从心脏、肝脏、脾脏、肺脏、肾脏、十二指肠、直肠、法氏囊、胸腺、胰腺、脑组织、胸肌、食道、腺胃、血液、舌头、口腔分泌物、皮肤、骨髓和粪便等20种组织器官中检测到DPV的DNA。检出时间最早和检出率最高的组织器官为肝脏和脑组织。结果为阐明DPV的致病机理和应用PCR方法检测感染鸭体组织中的DPV提供了重要的实验数据。更多还原

【Abstract】 In this paper, a pathology modei in which DPV were acutly infected was found when Duck Plague Virulent,SC1 Strains were subcutaneously injected into 56 adult Sichuan ducks,and the Polymerase Chain Reaction(PCR) was used to detect the distribution of DPV in every tissue or organ of infected duck at different time. The result indicate that the DPV DNA can be firstly detected 2 hours after inoculability from brain,liver,spleen,bursa of fabricius,thymus. 12 hours past, the DPV DNA can be detected from heart,liver,spleen, lung,kidney, duodenum, rectum, bursa of fabricius ,thymus, pancreas, brain, brisket, esophagus, stomach ,bJood, tongue , bucca! secretion, skin ,medulla and dejecta. The tissues which be detected the car;iest and the highest efficiency were the liver and brain.The result of the study will provide significant testing data for illuminating nosogenesis of DPV and detecting DPV by PCR in infected duck body.更多还原