【作者】 孟琼华； 程安春； 汪铭书； 朱德康； 刘维平； 黄诚； 宋涌；

【Author】 Meng Qionghua Cheng Anchun Wang Mingshu (Sichuan Agricultural University,Yaan,Sichuan,China,625014)

【机构】 四川农业大学动物科技学院；

【摘要】 应用鹅源血清1型鸭疫里默氏杆菌(RA)作为抗原免疫兔制备兔抗RA的IgG,建立了雏鹅感染鸭疫里默氏杆菌的间接免疫酶组织化学法(Indirect Immunoperoxidase Staining,IIS),该法与大肠杆菌(O1O8)、沙门氏菌、多杀性巴氏杆菌感染发病和死亡雏鹅组织不出现阳性反应而与RA感染发病和死亡雏鹅组织出现特异性阳性反应。RA人工感染雏鹅24小时即可在心脏、肝脏、脾脏、肾脏、回肠、直肠肺脏、十二指肠、空肠和盲肠检测到RA抗原,RA抗原分布于宿主细胞、组织间隙和血液,检测RA人工感染和临床病例与RA肝脏分离符合率为100%。该法具有特异、直观的特点,可用于雏鹅RA人工感染和临床感染的诊断、检测及RA感染后抗原定位和RA致病机理的研究。更多还原

【Abstract】 Geese were artificially infected with Remerella anatipestifer serotype l at 21 days old to study the morphological pathology and to establish indirect immunohistochemistry methods to detect RA antigen and RA antigen distribution in infected geese. The gross pathological changes were as follows: fibrins on the seromembrane of all the organs, especially on the heart, liver, air sac and meningitis; swelling of liver and thymus ,swelling of spleen and bursal of Fabricius in early period and atrophy in the later stage, hyperemia and hemorrhage of the intestine, aberration of cecum in the later stage. Many parenchyma organs of infected geese showed fibrins and necrosis inflammation. Intestine showed hemorrhage inflammation. Few lymphocyte was found in spleen, thymus, bursa of fabricius and cecal tosil. Neutrophils infiltration could be seen in most of the organs. Etablish indirect immunohistochemistry methods to detect RA antigen and RA antigen distribution in infected geese and founded that this method showed great specificity and sensitivity.The result of indirect immunochemistry demonstrated that: 24 hours PI, heart, liver, spleen, kidney, ileum, rectum, lung, duodenum and cecum were positive; 46 hours PI, all the organs showed positive results and some of the organs showed heterophile substances which could be stained by toluidine blue; 58 hours PI, the positive results were most obvious in all organs; 5 days PI, alveloar endothelial celis and hepatocytes showed positive in immunochemistry detection; 9 days PI, liver, kidney and thymus stili showed strong results in immunohistochemistry detection while that of other organs began to decline; jejunum and pancreas shovved less obvious results compared with other organs listed above in Immunohistochemistry detection and brain was the least obvious.In summary, RA infection in geese could cause severe damages to parenchyma organs, intestines and the immune organs; pathological lesions were correlated with the distribution of RA antigen and neutrophils.更多还原