【作者】 赵国琦； 王志跃； 贾青； 孙龙生； 丁健； Nakashima Y.；

【Author】 Zhao Guoqi Wang Zhiyue Jia Qing Sun Longsheng Ding Jian(Dept. of Ani. Sci., Ani. Sci. and Vet. Med. Coll., Yangzhou Univ., Yangzhou 225009)Nakashima Y. (Faculty of Agriculture, Iwate Univ. 025 Japan)

【机构】 扬州大学畜牧兽医学院动物科学系； Faculty of Agriculture,lwate Univ.025 Japan；

【摘要】 用4只装有瘤胃瘘管、十二指肠近端瘘管和回肠末端瘘管的去势(公母各半)萨福克羊(体重平均为45.5±5.2kg),在饲喂不同比例的鸭毛草R和特制精饲料(C)(R:C 10:0、7:3、5:5、3:7)的日粮条件下,对小肠内的瘤胃微生物(主要为细菌)氨基酸利用情况进行了研究。结果表明:1.瘤胃细菌所含标记物DAPA浓度在10:0～5:5日粮条件下很稳定(2.0mg/g·DM).3:7组则显著地升高(2.6mg/g ·DM);十二指肠和回肠内容物中DAPA浓度分别随着日粮精饲料水平提高而增大(分别为0.37～0.50、0.27～0.47mg/g·DM)。2.体重换算为40kg的绵羊每只每天进入十二指肠和回肠的DAPA量差异不显著,分别为0.16～0.19和0.06～0.07g:3.随着日粮中精饲料水平的提高,每100g细菌体中所含Cys、Met和Trp量显著地减少(p<0.05)(r=-0.99、-0.75、-0.66),DAPA量则显著地增加(p<0.05)(r=0.88)。4.随着日粮中精饲料水平提高小肠内被吸收的菌体氨基酸量在7:3～3:7组显著地减少(p<0.01),10:0组则低于7:3组而接近于5:5组。更多还原

【Abstract】 Four castrated Suffolk sheep (average body weight 45.5 ± 5kg) with rumen cannula, duodenum cannula, and ileum cannula were used to determine the utilization rate of amino acid from rumen bacteria in small intestine with different orchardgrass hay(R):concentrate(C) ratios (R:C 10:0 7:3 5:5 3:7).The result indicated that the contents of rumen bacteria 2,6-diaminopimeric acid (DAPA) were stable (2.0mg/g · DM) as the sheep were fed the dietary with R:C ratio 10:0-5:5 and increased significantly to 2.6mg/g -DM as R:C ratio was 3:7.The contents of DAPA reaching duodenum and ileum increased significantly as the level of dietary concentrate increased, they were 0.37-0.50,0.27-0.47mg/g -DM respectively. There were no clear differences between the amount of DAPA reaching duodenum and ileum every day in sheep based on 40kg body weight. They were 0.16-0.19, 0.06-0.07g/day respectively. As the level of dietary concentrate increased, the amount of Cys, Met and Trp in per 100 gram of total of bacteria reduced significantly (p<0.05)(r=0.88). As the level of dietary concentrate increased, the amount of amino acid from bacteria absorbed in small intestine decreased significantly (R:C 7:3-3:7), and those in dietary (R:C 10:0)were lower than in dietary (R:C 7:3), and closed in dietary (R:C 5:5).更多还原