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柔嫩艾美耳球虫TA4重组抗原的免疫试验
Vaccination against Coccidiosis with TA4 Recombinant Antigen of Emieria tenella
【作者】 张莉； 秦泽荣； 徐镔蕊； 何召庆； 王春凤； 刘尚高；
【Author】 Zhang Li, Qi Zerong, Xu Binrui, He Zhaoqing, Wang CHunfeng, Liu Shanggao (China Agricultural University, Beijing 100094. China)
【机构】 北京市农林科学院； 中国农业大学动物医学院；
【摘要】 构建能表达柔嫩艾美耳球虫TA4抗原的重组质粒.使其在乳球菌中。用刚出壳的健康雏鸡为实验对象.对乳球菌表达的TA4重组抗原进行研究。用表达球虫TA4抗原的工程乳球菌5×108个/只/天的剂量口服雏鸡10天,同时设置乳球菌组、未免疫未攻毒组和未免疫攻毒组作对照.于11日龄用5×104个强毒卵囊进行攻毒。结果表明TA4重组抗原组抗球虫指数为160.12.实验鸡可获得一定免疫保护力。
【Abstract】 To construct a recombinant vector that expresses TA3 antigen of Eimeria tenella in Lactococcus lactis against Coccidiosis. Biological activity of the recombinant antigen was studied by animal experiment. Young chickens were vaccinated in 10 days with engineering Lactococcus containing TA4 recombinant antigent at 5 × 108/chicken/day of dose. The groups of unvaccinated/unchallenged (uvuc) , unvaccinated/challenged (uvc) and live Lactococcus were set up as controls. Experimental birds were challenged with 5× 104 sporulated oocysts of E. tenella at 11 days of age. The result showed that TA4 recombinant antigen could induce a partial protection for chickens against E. tenella (ACI:160. 12).
【Key words】 Eimeria tenella； TA4 recombinant antigen； Lactococcus； vaccination；
- 【会议录名称】 中国畜牧兽医学会禽病学分会第十一次学术研讨会论文集
- 【会议名称】中国畜牧兽医学会禽病学分会第十一次学术研讨会
- 【会议时间】2002-10
- 【会议地点】中国四川成都
- 【分类号】S852.7
- 【主办单位】中国畜牧兽医学会禽病学分会（Poultry Health Branch, Chinese Association of Animal and Veterinary Science）