【作者】 鄢明华； 李秀丽； 丁伯良； 王英珍； 白朋勋； 黄金海； 赵相华；

【Author】 Yan Minghua, Li Xiuli, Ding Boliang, Wang Yingzhen, Bai Pengxun, Huang Jinhai, Zhao Xianghua (Tianjin Institute of Animal Husbandry and Veterinary Science, Tianjin, 300112)

【机构】 天津市畜牧兽医研究所；

【摘要】 从天津地区各区县养鸡场分离的经生化鉴定、动物回归试验获得45株鸡致病性大肠杆菌。对45株致病性大肠杆菌进行血清型鉴定.共定型出31株.分属14个血清型.其中O78,O88,O2,O45,O53,O14为优势血清型.占定型菌株的58.3%。提取O78(6株)、O88(3株)、O2(3株)3个血清型11个分离株的外膜蛋白(Outer membrane protein.OMP)测定其OMP型.SDS-PAGE分析表明这些分离株共产生了3个OMP型.OMP-1型由2条带组成.OMP-2型和OMP-3型均由3条带组成.但OMP-3型分子量最大的一条带的迁移率明显低于OMP-2型相应条带。OMP-1型和OMP-2型由O78,O68,O23个血清型所共有。OMP-3型为O78血清型所独有。这表明同一血清型的菌株可能属于完全不同的OMP型.而血清型不同的菌株也可能为同一OMP型更多还原

【Abstract】 45 strains of Pathogenic Escherichia coli(E. coli) were isolated from chicken farms in Tianjin region and were studied for O serotypes. Thirty one of them were identified as O type belonged to 14 serogroups predominant the O78,O88,O2,O45,O53 and O145. Outer membrane proteins (OMP) were prepared from 11 strains belonged to O78,O88 and O2 serogroups. SDS -PAGE analysis showed that 3 OMP patterns were found from these strains. OMP pattern consisted of two protein bands was named OMP-1 pattern. OMP - 2 and OMP - 3 pattern had of three protein bands and the largest protein band of OMP-3 pattern moved slower than that of the OMP -2. The OMP-1 pattern and OMP-2 pattern were found from all of the O78, O88 and O2 serogroups, but the OMP-3 pattern were only seen from O78 serogroups. These results showed that strains of different O serogroups of chicken E. coli could have the same OMP pattern and the strains belong to the same O serogroup could have different OMP patterns.更多还原