【作者】 张春杰； 李银聚； 程相朝； 吴庭才； 郑祥海；

【Author】 Zhang Chunjie,Li Yinju,Cheng Xiangchao et al. (Department of Animal science, science & technology university of Henan. Luoyang 471003)

【机构】 河南科技大学动科系；

【摘要】 从豫西地区发病鸡群中分离的鸡减蛋综合征病毒(EDSV),经非免疫鸡胚增殖后,用差速离心法纯化和蛋白酶K法抽提,得到全长约34.3kb的EDSV基因组。分别以Hind Ⅱ、BamHI、EcoRI及BamHI+EcoRI对EDSV-gDNA进行限制性酶切。分别得到9、4、4和7个片段。用1%琼脂糖凝胶电泳酶切样品并以凝胶成像系统对酶切片段的大小和分子量进行测定。将这些结果与NE4、WPDV205、AV-127标准株及B8/78株基因组DNA由相同酶切的结果进行比较发现.豫西地区EDSV与AV-127和B8/78较为相似,但也存在微小差别.与其他毒株的差别较大。更多还原

【Abstract】 Restriction endonuclease maps of egg drop syndrome virus (EDSV) isolated from the west region of Henan province was made by using four different restriction endonucleases Hind Ⅲ , BanHI, EcoRI and BamHI+EcoRI. It was found that the DNA fragments were 9,4,4 and 7, respectively. Compared with NE4, WPDV205, AV-127 and B8/78, there were some differences in restriction maps and fragments. It was proved that different EDSV strain had different DNA types.更多还原