【作者】 单松华； 邹键； 姚龙涛； 胡永强； 何水林； 龚祖埙；

【Author】 Shan Songhua, Zou Jian, Yao Longtao,Hu Yongqiang, Hu Suiling, Gong ZuXun(1 Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200031. China , 2 Institute of Biochemistry and Cell Biology, the Chinese Academy of Sciences, 3 Shanghai Fenxian Veterinary Station)

【机构】 上海出入境检验检疫局； 中国科学院上海生命研究院生物化学与细胞生物学研究所； 上海市奉贤畜牧兽医站；

【摘要】 对新近分离的鹅副粘病毒SF02株通过自行设计的三条引物建立了复合RT-PCR方法。该方法对鹅源副粘病毒SF02株、GPV2株可检测到215bp和401bp两条带.对新城疫病毒参考毒株F48E9、N79、Herts/33、Roakin、Komarov、Mukteswar、V4/66、新城疫野毒只出现一条401bp带.与预期的大小一致.对禽流感、传染性支气管炎病毒、SPF鸡尿囊液均未检测到特异性条带。本文首次报道采用复合RT-PCR区分鸡新城疫病毒与鹅副粘病毒。更多还原

【Abstract】 A multiplex RT -PCR technique has been developed for differentiation of goose paramyxovirus (GPV) and Newcastle disease virus. A set of three oligonucleotide primers(P3,P4 and P5) were designed and used in this multiplex RT -PCR technique of GPV and NDV genomic RNA. The RT -PCR product of 215bp and 401bp were generated only with RNA from SF02 and GPV2 isolate orginated from goose, whereas 401bp product only with RNA from reference strain NDV, F48E9, N79, Herts/33,Roakin ,Komarov,Mukteswar,V4 66 and field isolate NDV1 originated from chicken. However, the RT - PCR failed to detect avian influenza virus, infectious boncheitis virus and the allantoic fluid of SPF chicken. As far as we know, this is the first report on differentiation of GPV(genotype Ⅶ ) and NDV.更多还原