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鸽新城疫病原的分离鉴定及油佐剂灭活苗的研制
Preparation of Oil-adjuvant Inactivated Vaccine and Isolation and Identification of the Pathogens from Pigeons with Newcastle Disease
【作者】 符子华; 易忠; 成进; 麻迎东; 林福滨; 李令启; 沙依然古丽;
【Author】 Fu zihua ,Yi zhong ,Cheng Jin,Ma Yingdong ,Lin Fubin,Li Lingqi,Shayiran Guli(1. Veterinary Research Institute,Xin Jiang Academy of Animal Sciences, Urumqi 830000. China;2. General Veterinary Station of Epidemic Prevention and Surveillance of Xin Jiang )
【机构】 新疆畜牧科学院兽医研究所/新疆天康畜牧生物技术股份有限公司生物制品部; 新疆动物防疫监督总站;
【摘要】 应用SPF鸡胚从送检发病鸽的脑、脾脏组织分离到6株病毒.结合流行病学、临床症状、病理解剖变化、病原分离、回归鸽试验和HA与HI血清学试验证明近年来我区鸽群广泛流行鸽新城疫。利用当地分离的鸽新城疫病毒研制出鸽新城疫油佐剂灭活苗.疫苗接种试验鸽无任何不良反应.免疫后20天抗体水平达到高峰.强毒攻击的保护率为100%.抗体水平在5log2以上可维持120天以上.表明鸽新城疫病毒油佐剂灭活苗可使鸽产生较高水平的抗体.有较长的免疫持续期。
【Abstract】 6 virus strains were isolated from the brain and spleen of sick pigeons using SPF chicken embryos. It is proved that Pigeons with Newcastle disease has been very polular in Xin Jiang in recent years through the analysis of epidemiology, clinic symptoms, gross lessions the results of pathogen isolation, reversion test and serological test of HA and HI. The oil ?adjuvant inactivated vaccine virus was prepared with NDV isolated from local pigeons. The results showed that the vaccine had no side effect after used on trialed pigeons. The level of its antibody reached the peak at 20 days after vaccination and its protection rate was 100% when pigeons were challenged with the local virus. It still kept the level of antibody over 5 log2 after 120days of vaccination.
【Key words】 Pigeon Newcastle disease; isolation and identification; oil- adjuvant inactivated vaccine;
- 【会议录名称】 中国畜牧兽医学会禽病学分会第十一次学术研讨会论文集
- 【会议名称】中国畜牧兽医学会禽病学分会第十一次学术研讨会
- 【会议时间】2002-10
- 【会议地点】中国四川成都
- 【分类号】S852.65;S859.797
- 【主办单位】中国畜牧兽医学会禽病学分会(Poultry Health Branch, Chinese Association of Animal and Veterinary Science)