【作者】 朱道中； 吴红专； 刘福安； 贺东生； 李惠颜；

【Author】 ZHU Daozhong, WU Hongzhuan, LIU Fuan, HE Dongsheng, LI Huiyan (Department of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642)

【机构】 华南农业大学动物医学系；

【摘要】 鸡传染性喉气管炎病毒(ILTV)胸苷激酶(TK)基因插入缺失毒株是由我校研制的国内首次构建的ILT基因工程疫苗候选株。本研究的目的在于检测其对鸡的安全性和免疫效力。结果表明:ILTV TK基因插入缺失株能够在鸡喉气管上皮内增殖,并形成典型的核内包涵体;随着日龄的增加,科宝—500白羽肉鸡对ILTV TK插入缺失毒株的易感性增强;免疫接种时最好采用点眼滴鼻途径,鸡在接种ILTV TK插入缺失毒株后,能够产生相应的抗体,并于接种后第3周抗体IgG首次达到最高水平,但抗体IgG水平的高低与免疫保护率没有明显的相关性。更多还原

【Abstract】 A thymidine kinase(TK) gene insertion-deleted strain of avian infectious laryngo-tracheitis virus (ILTV), the first genetically engineered vaccine strain candidate for ILT control in our country was developed at our university. This study was to examine its safety and immune efficacy for chickens. The results of the experiments showed that the ILTV TK gene insertion-deleted strain could propagate in the avian laryngotracheal epithelium and form pathogenic intranuclear inclusion bodies; older Cobb-500 white broilers were more susceptible to most of the ILTV strains than younger birds; the best way to vaccinate the birds was by ocular/nasal instillation ; chickens vaccinated with the ILTV TK gene insertion-deleted strain could elicit a correlative immune response, and the IgG level peaked at 3 weeks post-vaccination after primary immunization , but the antibody liter had no apparent correlation with the protection rate.更多还原