【作者】 肖虎； 王德昌； 霍然； 冷向峰； 冯永强；

【Author】 Xiao Hu, Wang Dechang, Huo Ran, Leng Xiangfeng, Feng Yongqiang Shandong University Shandong Provincial Hospital, Ji’nan 250021

【机构】 山东大学医学院山东省立医院；

【摘要】 <正>目的:研究严重烫伤休克期大鼠大鼠淋巴管运动变化及淋巴液中TNF、IL-6、IL-8的水平的变化,观察肠系膜淋巴管内皮细胞超微结构变化。方法:将42只成年雄性Wistar大鼠随机分为A烧伤补液组(18只)和B烧伤非补液组(18只)、C对照组(6只)。在A组和B组动物背部造成30%TBSAⅢ度烫伤,A组腹部皮下注射生理盐水(NS)更多还原

【Abstract】 Object: To studythe dynamic changes of lymphatic motion and ultrastructural changes of lymphatic endothelium, and measure tumor necrosis factor (TNF), interleukin-6 (IL-6), interleukin-8(IL-8) levers in lymph of bum shock rats. Method: 42 male adult Wistar rats were randomly divided into the bum resuscitation group (group A,n=18), the burn non- resuscitation group (group B.n=l 8) and the control (group C, n=6). Using inverted microscopy and digital image processing to observe the motive frequency of the rat mesenteric lumphatic vessels and measure the speed of lymph and the concentrations ofTNF, IL-6,IL-8 in lymph at 6,24,48 hours post burn. Results: In group A and B, the contractile frequency of the mesenteric lymphatic vessels were decreased, together with ultrastructural disruptions such as swellon and contacts disruption of lymphatic endothelium, despite the increasing lymph speed ,and the levers of TNF,IL-6,IL-8 in lymph were increased compared with group C. Conclusion: In the burn shock stage, the motion of the lymphatic vessles were decreased together with ultrastructural disruptions in lymphatic endothelium.and the levers ofTNF, IL-6, IL-8 in lymph were increased.Fluid resuscitation could improve the lymph circulation and decrease the levers of TNF, IL-6,IL-8 in lymph.更多还原