【作者】 何永红； 唐永红； 谭石慈； 邢达；

【Author】 Yonghong He Yonghong Taiig Shici Tan Da Xing Institute of Laser Life Science, South China Normal University, Guangzhou, 510631

【机构】 华南师范大学激光生命科学研究所；

【摘要】 光动力学诊断从原理上不可克服自体荧光干扰。激发效率低等限制,针对这一现状,我们首次提出并设计了一种新的肿瘤诊断方法。光敏剂HpD首先选择性地在肿瘤组织内积聚,再利用长波长红光激发HpD产生光敏作用,结果是在肿瘤组织内产生大量单线态氧但并不产生自体荧光,利用FCLA和单线态氧反应产生化学发光(其峰值波长在532nm),再利用高灵敏度探测器检测此发光的二维分布,便可以达到肿瘤定位的目的。我们从实验上证明了FCLA辅助的光动力学诊断方法的可行性。如改用红区主吸收的光敏剂,并提高激发强度,这种方法可望在临床肿瘤诊断上得到实际应用。更多还原

【Abstract】 Conventional Photo-Dynamic Diagnosis (PDD) has several serious limitations, such as autofluorescence disturbance, poor penetration ability of the exciting laser etc. All these problems are because the exciting laser wavelength at the main absorption peak of the photosensitizer (HpD) is too short. But to our knowledge, long wavelength He-Ne laser can sensitize HpD to produce singlet oxygen (1O2) without autofluorescence of the normal tissues. The O2 can react with FCLA (5-Fluoresceinyl Cypridina Luminescent Analog) to emit chemiluminescence at the peak wavelength 532nm, thus we can use a high sensitivity detector to image the emission from FCLA to make a localization of the cancer. This is a novel idea proposed by our group, and its feasibility was proved by our experiments.更多还原