【作者】 吴云锋； 顾沛雯； 安凤秋； 相建业； 罗朝鹏； 杨英；

【Author】 WU Yun-feng,GU Pei-wen,AN Feng-qiu,XIANG Jian-ye,LUO Zhao-peng,YANG Ying(College of Plant Protection,Northwest A & F University,Shaanxi Key Laboratory for Agriculture Molecular Biotechnology,Yangling,Shaanxi 712100,China)

【机构】 西北农林科技大学植保学院陕西省农业分子生物重点实验室；

【摘要】 小麦蓝矮病是陕西乃至西北冬麦区的一个重要病害,由介体条沙叶蝉专化性传播。利用植原体16SrDNA基因保守序列通用引物对Rm16 F2/Rm16 R1,应用PCR技术从小麦蓝矮病株叶片中扩增到1.4kb的特异片段。通过介体条沙叶蝉对小麦蓝矮病植原体寄主范围进行研究,在接种的18种寄主植物中,有8种寄主表现症状,表明小麦蓝矮病植原体寄主范围广泛。更多还原

【Abstract】 Wheat blue dwarf(WBD)is an important disease of winter wheat disseminated by Psammotettixstriatus L.in Shaanxi and Northwest area.A DNA fragement 1.4 kb were amplified by polymerasechain reaction with primers(Rm16 F2/Rm16 R1)for phytoplasma 16S rDNA and DNA samples extractedfrom infected wheats but not in those extracted from healthy plants.Host ranges reach up to 8 speciesthrough inoculation of 18 different hosts in greenhouse.It shows that wheat blue dwarf phytoplasma haswide host ranges.更多还原