【作者】 蔡为明； 冯伟林； 金群力； 范丽军； 吴永志；

【Author】 CAI Wei-ming FENG Wei-lin JIN Qun-li FAN Li-jun WU Yon-zhi (Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou,310021)

【机构】 浙江省农业科学院园艺研究所；

【摘要】 本试验以双环蘑菇Agaricus bitorquis、金针菇Flammulina velutipes和杏鲍菇Pleurotus eryngii 3种食用菌菌丝为材料,采用60Coγ射线进行了200gy、500gy、800gy 3个不同剂量的辐射处理,并开展了菌丝生长试验、电导率测定和ISSR分析,研究结果表明:随着辐射剂量的增加,食用菌菌丝生长受抑制增强,双环蘑菇菌丝对60Coγ辐射最为敏感,当辐射剂量增强到800Gy时,双环蘑菇菌丝失去活力;经非致死剂量辐射的3种食用菌菌丝均随着继代培养次数的增加,其萌发和生长速度加快,生长势增强,活力逐渐恢复。3种食用菌菌落经辐射处理后,其浸出液的电导率均随着辐射剂量的加大而增加,当金针菇和杏鲍菇菌落的辐射剂量为500Gy、双环蘑菇辐射剂量为200Gy以上时电导率显著高于对照。ISSR分析结果表明,3种食用菌菌丝的各辐射处理均与对照存在不同程度的多态性差异,经辐射处理后多态性条带数量发生变化,出现了特异性条带,既有新增的条带,又有缺失的条带。其中双环蘑菇变异最大,200gy和500gy辐射引起的ISSR变异率分别为33.3%和50.5%;金针菇变异最小,200gy、500gy和800gy辐射引起的ISSR变异率分别为2.3%、2.3%和3.1%;200gy、500gy和800gy辐射杏鲍菇菌丝引起的ISSR变异率分别为8.6%、13.4%和20.0%。更多还原

【Abstract】 Mycelia of Agaricus bitorquis,Flammulina velutipes and Pleurotus eryngii were treated with ⁶⁰Co y-irradiation at the doses of 0,200,500 and 800Gy.The results showed that irradiation inhibited their mycelia growth positively.Of the three edible mushroom,Agaricus bitorquis was the most sensitive one to irradiation,its mycelia were die at the dose of 800Gy.Germination rate,growth rate and vigor of all mycelia irradiated with un-lethal doses increased along with the times of transfer of culture.Electrical conductivity analysis showed that irradiation increased EC of leaching solution of mycelia positively.The EC of leaching solution of mycelia of Flammulina velutipes and Pleurotus eryngii irradiated with 500Gy,Agaricus bitorquis irradiated with 200Gy, were significantly higher than that of 0Gy treatment ck.ISSR analysis revealed that ⁶⁰Coγ-irradiation effect on DNA variation of Agaricus bitorquis,Flammulina velutipes and Pleurotus eryngii mycelia in varying degrees. Number of polymorphic bands was changed after irradiation.Flawing bands and increasing bands were observed in amplified bands of 14 selected ISSR primers.The ISSR variation rate of Agaricus bitorquis for 200gy and 500gy treatments was 33.3%and 50.5%;The ISSR variation rate of Flammulina velutipes for 200gy,500gy and 800Gy treatments was 2.3%,2.3%and 3.1%,that of Pleurotus eryngii was 8.6%,13.4%and 20.0%.更多还原