【作者】 杨静； 李成云； 刘林； 李进斌； 周晓罡； 业艳芬；

【Author】 YANG Jing, LI Cheng - yun, LIU Lin, LI Jin - bin, ZHOU Xiao - Gang, YE Yan-fen (The Ministry of Education Key Laboratory for Agricultural Biodiversity For Pest Management, Yunnan Agricultural University, Kunming, 650223 China; Biotechnology Research Institute of Yunnan Academy of Agricultural Science; Plant Protection Research Institute of Yunnan Academy of Agricultural Science, Kunming, 650205)

【机构】 云南农业大学教育部生物多样性与病虫害控制重点实验室； 云南省农业科学院植物保护研究所； 云南省农业科学院生物技术研究所；

【摘要】 拟南芥基因组是第一个完成测序的植物基因组。目前,拟南芥分泌蛋白组的预测及功能的分类还没有系统报道, 本文就拟南芥分泌蛋白组的预测及功能分类进行初步研究。组合的分泌蛋白预测软件SignalP v3．0、TargetP v1．01、big-PI predictor和TMHMM v2．0被用于拟南芥分泌组的预测,得到828个“典型”的分泌蛋白,占拟南芥基因组的2．86％。采用 SubLoc v1．0对282个分泌蛋白的亚细胞位置进行了预测,结果表明,分泌的亚细胞位置有细胞质、细胞外、线粒体和细胞核, 大部分分泌蛋白的亚细胞位置是细胞外, 而少数的亚细胞位置是细胞质、线粒体和细胞核。由于某些运送至线粒体、细胞质或细胞核的蛋白的定位与这些场所代谢产生的能量或物质有关,因此,这类少数的蛋白仍属于分泌蛋白。根据基因功能分类表对拟南芥基因组中编码282个分泌蛋白的基因功能进行了分类,大部分编码分泌蛋白的基因主要参与代谢、细胞运送、细胞修复与防御、蛋白寿命和贮藏蛋白。同时分析了具有细胞修复与防御功能的编码分泌蛋白的基因主要具有过氧化物酶、激酶、抗性蛋白、富含亮氨酸重复蛋白和病程相关蛋白五种功能,占所有参与细胞修复与防御的分泌蛋白的50．79％。具有代谢功能的编码分泌蛋白的基因主要具有水解酶、脂肪酶、羧肽酶、转化酵素、转移酶、伸展蛋白和合成酶等七种功能,占具有此类功能的编码分泌蛋白基因的70．33％。本文还初步比较了拟南芥、人类及老鼠编码分泌蛋白基因的功能的异同,发现外源凝集素、富含亮氨酸蛋白、蛋白酶抑制子、细胞色素、防卫素、肽酶和蛋白酶是拟南芥、人类及老鼠编码分泌蛋白的基因所共有的功能,编码几丁质酶的基因是拟南芥所特有的,而编码趋化因子和转化生长因子的基因是老鼠和人类特有的。通过相关生物信息学软件对拟南芥分泌蛋白组预测及功能的分类,将加速实验室对南芥分泌蛋白组功能的研究。更多还原

【Abstract】 Arabidopsis thaliana is the first plant genome that has been sequenced completely. At present, secretome of Arabidopsis thaliana and their gene function categorization have not been systematically reported. Here elementally reported the prediction of secretome of Arabidopsis thaliana and function categorization of gene encoding secretory protein. Combined the computer - based software such as SignalP v 3.0, TargetP v1 .01, big - PI predictor and TMHMM v 2.0 were used to predict the secretome of Arabidopsis thaliana. The result showed that 282 secretory proteins within 28953 proteins were obtained, the proportion of predicted secretory proteins to total proteins in entire genome was 2.86%. And sublocalization of 282 secretory proteins were further predicted through SubLoc v1 .0, their sublocaliza-tions wen cytoplasmic, extracellular, mitochondrial and nuclear, most of them were secreted into extracellular space, while a few of them were secreted into the other three sublocalizations, but they were not excluded from secretory proteins, because these proteins, which were transport into cytoplasmic, mitochondrial and nuclear, were further secreted into extracellular space by the aid of energies and related materials produced by gome metabolism in three organelles. The most of genes encoding secreted proteins participated in cell metabolism, cell rescue and defense, cell transport, cell fate and storage protein. Among the genes participated in cell rescue and defense, the ratio of genes that were with functions of peroxidase, kinase, disease resistance protein, pathogenesis - related protein and leucine - rich repeat protein was 50.79%, at the same time, of the genes participated in cell metabolism, the ratio of genes with functions of hydrolase, li-pase, carboxypeptidase, invertase, transferase, expansin and synthase was 70.33%. Functions of some genes encoding secretory proteins in Arabidopsis thaliana, mouse and human genome were compared. The result demonstrated that lectin, leucine - rich repeat protein, protease inhibitor, cytochrome, defensin, peptidase and protease were common in three genomes, while genes encoding chemokines and transforming growth factors existed in mouse and human genomes, the gene encoding chitinase was only present in Arabidopsis thaliana genome. Prediction of Arabidopsis thaliana secretome by the aid the combined computer - based software will accelerate the experimentally functional study of secretome.更多还原