孢子丝菌分子生物学DNA多态性分型研究

【作者】 罗庆录； 李莉莉；

【机构】 北华大学附属医院；

【摘要】 目的:研究孢子丝菌DNA分型,并探讨DNA分型技术在临床菌钟鉴定以及流行病学中的应用。方法:本实验采用氯化苯法提取DNA,以NS₅、NS₆为引物用随机扩增DNA多态性（DAPD）方法对临床分离的30株孢子丝菌进行DNA分型。结果:30株孢子丝菌的DNA带型不完全相同,具有一定的遗传变异性。30株临床分离的孢子丝菌可进一步分为4型,不同部位取材的菌株间及临床型不同的患者的菌株间DNA型基本相同。结论:随机扩增DNA多态性研究发现孢子丝菌具有一定的种内差异,该方法可将临床分离的孢子丝菌分为4型,本研究对病原学诊断探讨感染来源,传播途径等流行病学研究奠定了基础。更多还原

【Abstract】 Objective: TO investigate the DNA type of Sporothrix Schenckii and to discuss the application ofDNA typing method in its clinical identification and epidemiology study. Method: DNA extraction was carriedout by using benzyl chloride, the primers,including NS5.NS6 were used in this test .and random amplification ofpolymorphic DNA(RAPD) assay was employed for DNA typing of 30 clinical isolates of Sporothrix SchenkiiResults: The RAPD patterns of 30 isolates showed some degrees of variation. Thirty clinical isolates of S.Schenckii were divide into 4 types, and showed high similarity one another. Identical DNA pattern was found instrains from different sites of inferent in the same patient, and even from different patients Conclusions: Thereare some genenic variations between 30 isolates of S. Schenckii. The clinical isolates can be divided into 4 typesby RAPD, and showed high similarity one another. This experimental has great significance in the pathogenicdiagnosis and epidemiology study to investigate the source of infection and transmission of pathogens and otherepidemiology study.更多还原