【作者】 杨晓彤； Jennifer M.F.Wan； 糜可； 冯慧琴； Daniel K.O.Chan； 杨庆尧；

【Author】 Yang Xiao-Tong, Jennifer Man-Fan Wan, Mi Ke, Feng Hui-Qing, Daniel K. O. Chan, Yang Qing-Yao( Department of Zoology, Faculty of Science, The University of Hong Kong, Hong Kong SAR, China; The Institute of Microbiology and Immunology, Shanghai Teachers University, Shanghai 200234, China)

【机构】 香港大学理学院动物学系； 上海师范大学微生物与免疫学研究所；

【摘要】 <正> (1→3)-□-glucan is a core structure in mushroom polysaccharide, which claim to posses anticancerous and immunomodulatory properties. The ability to identify (1→3)-□-glucan in mushroom polysaccharide not only provide useful information on the structural composition of the mushroom polysaccharide, but facilitate us to identify the potential anticancerous and immunomodulatory active compounds.Using limulus factor G test, we detected (1→3)-□-glucan in 27 polysaccharides extracted from 19 edible or medicinal mushrooms species. The result show that (1→3)-□-glucan exists in all mushroom polysaccharide extracts but its content varies extremely depends on the mushroom species or the part of mushroom and the degree of purification. Our data show the mean of (1→3)-□-glucan in these mushroom polysaccharides is 34.8%. Nine mushroom polysaccharide extracts from L. edodes, S. commune, C. versicolor, V. volvacea, C. comatus, G. frondosa, L. shimeji have superior (1→3)-□-glucan contents than the others. Our study demonstrates that limulus Factor G test is a quick and convenient method for detecting (1→3)-□-glucan content in crude mushroom polysaccharide extracts.更多还原

【Abstract】 (1→3)-□-glucan is a core structure in mushroom polysaccharide, which claim to posses anticancerous and immunomodulatory properties. The ability to identify (1→3)-□-glucan in mushroom polysaccharide not only provide useful information on the structural composition of the mushroom polysaccharide, but facilitate us to identify the potential anticancerous and immunomodulatory active compounds.Using limulus factor G test, we detected (1→3)-□-glucan in 27 polysaccharides extracted from 19 edible or medicinal mushrooms species. The result show that (1→3)-□-glucan exists in all mushroom polysaccharide extracts but its content varies extremely depends on the mushroom species or the part of mushroom and the degree of purification. Our data show the mean of (1→3)-□-glucan in these mushroom polysaccharides is 34.8%. Nine mushroom polysaccharide extracts from L. edodes, S. commune, C. versicolor, V. volvacea, C. comatus, G. frondosa, L. shimeji have superior (1→3)-□-glucan contents than the others. Our study demonstrates that limulus Factor G test is a quick and convenient method for detecting (1→3)-□-glucan content in crude mushroom polysaccharide extracts.更多还原