【作者】 覃宏涛； 肖晗； 孙宗修； 徐同；

【Author】 Qin Hongtao Xiao Han Sun Zongxiu Xu Tong( 1 Department of Plant Protect, Zhejiang University, I langzhou 310029 ) ( 2 Rice Biology Lxab of Agriculture Department, Rice Research Institute of China, I langzhou 310006 )

【机构】 浙江大学植保系； 中国水稻研究所农业部水稻生物学重点实验室；

【摘要】 以农杆菌介导法将木霉内切几丁质酶基因ThEn-42导入粳稻台北-309和农虎6号中。PCR检测表示ThEn-42已整合入水稻基因组中。在温室中进行的抗病性检测结果表明:T₀代转基因植株对纹枯病菌（Rhizoctonia solani Kühn）的抗性得到了明显的提高。T₀代转墓因植株的离体叶片也表现出对纹枯病菌的抗性。更多还原

【Abstract】 By an AgrobacLerium - mediated transformation method, TliEn - 42, an cndochitinasc gene cloned from Trichoderma harzianum strain P1, was introduced into the Japonica rice varieties Taibci-309 and Nonghu-6. About 75% of the regenerated transgcnic plant (T0) showed significantly higher resistance a-gainst the sheath blight pathogen Rhizoclonia solani Kuhn in greenhouse. Leaf of most TO transgcnic plants also showed less susccptbility to Rhizoctonia solani Kiihn in the in uiiro testing.更多还原