【作者】 王金祥； 蔡小杰； 廖红； 严小龙；

【Author】 WANG Jin-xiang, CAI Xiao-jie, LIAO Hong,YAN Xiao-long(College of Resource and environment, Root Biology Center, South China Agricultural University, Guangzhou 510640, China; College of Biotechnology, South China Agricultural University,Guangzhou 510640,China)

【机构】 华南农业大学资源与环境学院华南农业大学根系生物学研究中心； 华南农业大学生物技术学院；

【摘要】 以大豆下胚轴插条作为实验材料,探讨了植物激素生长素和乙烯对其不定根形成的影响。结果表明:吲哚乙酸(IAA)促进大豆下胚轴插条不定根的形成,以200μmol/LIAA效果最好。乙烯释放剂-乙烯利促进大豆插条的生根,尤其是100μmol/L乙烯利。乙烯合成抑制剂-氨氧乙酸(AOA)抑制大豆插条不定根的形成,乙烯合成前体,氨基环丙烷羧酸(ACC)促进不定根的形成,因此乙烯促进大豆插条不定根的形成。大豆插条离体后0～24 h对乙烯利作用敏感。乙烯对IAA促进生根起协同作用,IAA促进生根的作用至少部分依赖于乙烯。更多还原

【Abstract】 Soybean hypocotyl cuttings were employed to investigate the effects of auxin and ethyleneand their interactions on adventitious rooting. Our results demonstrated that auxin and ethylene stimulated adventitious rooting, especially at the levels of 100 μmol/L ethephon or 200 μmol/L IAA. Ethylene inhibitor (AOA) suppressed adventitious rooting and ethylene precursor (ACC) stimulated adventitious rooting. The period of 0-24 h after excision was very sensitive to the stimulatory effect of ethephon. Ethylene had a synergic role with IAA on adventitious rooting while the promoting effect of IAA was at least partially dependent on ethylene.更多还原