【作者】 郭祯； 陈兆安； 虞星炬； 金美芳； 刘远； 张卫；

【Author】 GUO Zhen CHEN Zhao-an, YU Xing-ju, JIN Mei-fang, LIU Yuan, ZHANG Wei (Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023,China;Graduate School of the Chinese Academy of Sciences, Beijing 100039,China;College of Marine Environmental Engineering, Dalian Fisheries University, Dalian 116023, China)

【机构】 中国科学院大连化学物理研究所海洋生物产品工程组；

【摘要】 培养阶段的藻不经浓缩直接用于产氢可显著降低光生物制氢的成本,考察了亚心形扁藻在4种培养基 (康维方培养基、海洋三号培养基、TAP培养基和本实验室的优化培养基)中生长动力学及暗诱导24 h后,在15 μmol／L解偶联剂CCCP(羰基氰化物间氯苯腙)作用下直接光照产氢过程。结果表明:优化培养基培养效果最好, 12 d细胞密度倍增10倍,比生长速率为0．192 d-1。康维方培养基培养的藻细胞直接产氢效果最好,细胞密度为 3×1096cells／mL的500 mL藻液,连续光照24 h产氢量达4．2 mL。营养盐及产氢工艺条件的优化对实现亚心形扁藻高密度培养直接光照产氢有重要影响。更多还原

【Abstract】 Significant cost-saving can be achieved in photobiological hydrogen production by green microalgae if the algae in the growth medium could be directly used for hydrogen production without the concentration and separation of algal biomass. The growth kinetics of a marine green algae Platymonas subcordiformis in four different culture media (Walne medium, marine No3 medium, TAP medium, an optimized medium) were investigated. The algal cells in the spent growth media were subjected to 24 h dark anaerobic induction and directly used for photobiogical hydrogen production with an uncoupler 15 μmol/L carbonylcyanide-m-chlorophenylhydrazone (CCCP). Results showed that the best growth was obtained in the optimized medium from our laboratory, with a specific growth rate 0.192 d-1. The highest photobiological hydrogen production was obtained for the cells grown in walne medium. About 4.2 mL hydrogen was produced at 3×106 cells/mL for 24 h in a 500 mL photo-bioreactor. The medium micronutrients have a significant impact on achieving high density cell culture and the yield of photobiogical hydrogen gas production in the spent growth media.更多还原