【作者】 李永峰； 任南琪； 李建政； 陈瑛； 朱葛夫； 刘敏； 邢德峰； 刘蕾； 王文静；

【机构】 哈尔滨工业大学市政环境学院；

【摘要】 厌氧产氢细菌的分离纯化与培养是发酵法生物制氢技术的基础课题之一,现有细菌的厌氧分离制备技术和培养基都存在着操作繁琐和成分复杂问题。通过厌氧培养技术比较和细菌生长试验,确定了“煮沸吹氮去氧,固液交替分离”的厌氧产氢细菌分离纯化培养改良Hungate技术与宽体窄颈培养瓶平板技术。通过培养基组成成分的增减和细菌增长、种类和数量的研究确立了厌氧发酵产氢细菌的分离和富集培养基。利用这些厌氧培养技术,已成功分离培养了550余厌氧菌株系。更多还原

【Abstract】 Isolation and culture of hydrogen-producing and fermentative bacteria is an important foundation on biohydrogen production process. There are complicated operation and composition in present anaerobic techniques and culture media. Hungate technique was improved and plate of culture bottle was established by comparing anaerobic methods and bacterium growth. Isolation and enrichment culture media were confirmed by the test of different composition and the species and amount of hydrogen-producing and fermentative bacterium. 550 bacterium strains were isolated by the anaerobic operation.更多还原