【作者】 王相晶； 任南琪； 李建政； 张颖； 陈兆波； 郭婉茜； 李永锋；

【机构】 哈尔滨工业大学市政环境工程学院；

【摘要】 采用间歇实验,研究了葡萄糖浓度、菌龄、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49（AF481148 in EMBL）生物产氢的影响。菌龄、接种量影响B49的产氢;B49生长和最适产氢所需的温度基本一致,均为35℃;B49不能利用无机氮源,有机氮是B49生长、产氢的适宜氮源;葡萄糖是B49发酵产氢的最适宜底物,当浓度为10g/L时,B49的葡萄糖利用率为100%,氢气产率为1.69mol H₂/mol葡萄糖;此外,B49可利用小麦、大豆、玉米、土豆及糖蜜废水和啤酒废水产氢,其中利用糖蜜废水、啤酒废水产氢分别为137.9mL H₂/g COD和49.9mL H₂/g COD。更多还原

【Abstract】 Batch experiments were conducted to investigate effects of glucose concentration, preculture time, inoculation volume, temperature, nitrogen source and various organic substrates on hydrogen production by a novel fermentative hydrogen-producing bacterial strain B49 (AF481148 in EMBL). Preculture time and inoculation volume could affect hydrogen production by B49. The optimum temperature for cell growth and hydrogen production was 35℃. B49 could produce hydrogen not from inorganic nitrogen source but from organic nitrogen source. Glucose was the best substrate for B49 to produce hydrogen. Glucose consumption and hydrogen yield for B49 with 10g/L glucose were 100% and 1. 69mol H2/mol glucose, respectively. In addition, B49 could generate hydrogen from other organic substrates such as wheat, soybean, corn, potato, molasses and brewage wastewater. Hydrogen yields from molasses wastewater and brewage wastewater were 137. 9 mL H2/g COD and 49. 9 mL H2/g COD, respectively.更多还原