Mechanism employed by Trichoderma koningii Smf2 against plant bacterial pathogen Ralstonia solancearum: Isolation, identification and antibiotic effect of peptaibols Trichokonin

【作者】 沈庆涛； 宋晓妍； 陈秀兰； 孙彩云； 谢树涛； 张玉忠；

【Author】 Shen Qing-tao Song Xiaoyan Chen Xiulan Sun Cayun Xie Shutao Zhang Yuzhong (State Key Laboratory of Microbial Technology, Shandong University, Ji’nan 250100, P.R.China)

【机构】 山东大学微生物技术国家重点实验室；

【Abstract】 The use of Trichoderma for biological purposes has become an effective alternative to control fungal plant pathogens, However, in the past few years, there have been little clear examples of Trichoderma used to control bacterial plant pathogens. Trichoderma koningii Smf2 was chosen from eight Trichoderma strains for its thermostatic metabolites with antibiotic activity against bacterial plant pathogen Ralstonia solancearum Smith. Exclusion chromatography (LH20) was used twice to partially purify targeted metabolites combined with biological test. LC/ESI-MS, a powerful tool for rapid identification and sequence determination of peptides, identified these metabolites as three peptaibols named Trichokonin Ⅵ, Ⅶ and Ⅷ, and their sequences were confirmed with NMR. Field experiment showed that 70%~80% of the wilting disease of ginger resulted from R. a solancearum Smith was suppressed. It was resulted that not only could Trichoderma effectively control the plant fungal pathogens, but also has good potential in biocontrol of the plant bacterial pathogens.更多还原