【作者】 励建荣； 于平；

【Author】 Li Jianrong Yu Ping (College of food science,Biotechnology and Environmental Engineering,Zhejiang Gongshang University,Food Safety Key Lab of Zhejiang Province,Hangzhou,310035)

【机构】 浙江工商大学食品生物与环境工程学院浙江省食品安全重点实验室；

【摘要】 探讨了从螺旋藻中分离纯化藻蓝蛋白的的优化条件。结果表明：钝顶螺旋藻细胞用 600W 的超声波处理25min,细胞已经基本破碎,之后用饱和度为55%的硫酸铵盐析,所得沉淀透析后再经过两次羟基磷灰石柱层析,最后获得的藻蓝蛋白得率为2．75%,二次纯化后得到的藻蓝蛋白的最高纯度为4．13。更多还原

【Abstract】 The optimal conditions for isolation and purification of phycocyanin from Spirulina maxima was studied.The results indicated that the cells of Spirulina maxima were basically broken after treated for 25min by ultrasonic,and then the salting-out was made with ammonia sulfate with saturation of 55% and the precipitation obtained was treated by quadratic hydroxylapatite exchanging chromatography,the final productivity and the highest purified fold of phycocyanin was 2.75% and 4.13,respectively.更多还原