【作者】 赵振刚； 于淑娟； 孔凡利； 秦慧民；

【Author】 Zhao Zhen-gang Yu Shu-juan Kong Fan-li Qin Hui-min (School of Light industry and Food Science,South China Univ.ofTech.,Guangzhou 510640)

【机构】 华南理工大学轻工食品工程学院；

【摘要】 建立了利用反相高效液相色谱同时测定甘蔗中10种酚酸的检测方法。采用 XTerra RP18色谱柱(6mm×150mm ID,5．0μm),以乙睛：水(含2%冰醋酸)为流动相,梯度洗脱,流速为1．0ml/min,28℃柱温,紫外检测器检测波长280nm。10种酚酸的回收率均在 85．45%～109．41%之间,相对标准偏差均低于4%。更多还原

【Abstract】 An analytical method of 10 phenolic acids in sugarcane was established by PR-HPLC. Chromatographic separation was performed by XTerra RP18 column(6mm×150 mm ID,5.0 μm)using the mobile phase gradient elution program.The eluant was Acetonitrile/H2O(2% HAC) with a flow rate 1.0ml/min at 28℃,the ultraviolet detection wavelength was 280 nm.The recovery rate was in the range of 85.45%～109.41%,and the RSD was less than 4% for 10 phenolic acids.更多还原