【作者】 帅益武； 尤玉如； 袁海娜；

【Author】 ShuaiYi Wu~1 You YuRu~(2*) Yuan Haina~2 1.ZheJiang Xiao WangZi Food Co.LTD,HangZhou LinAn,311300,China; 2.School of Biological and Chemical Engineering,Zhejiang University of science and technology,HangZhou 310012,China)

【机构】 浙江小王子食品股份有限公司； 浙江科技学院生物与化学工程学院；

【摘要】 以干制五倍子为原料,采用L16（4⁴）正交试验的方法,详细分析了鞣质溶剂浸提的主要影响因素,并通过溶剂萃取、Ca²⁺离子沉淀、明胶沉淀等不同分离纯化方法对鞣质最终得率和纯度进行了比较研究,初步建立了五倍子鞣质的提取工艺。实验结果表明：以100%丙酮为提取溶剂,温度50℃,pH2．0,时间10min 为五倍子鞣质的最佳提取条件,采用乙酸乙酯萃取的鞣质最终得率较高,且鞣质纯度达到了89．15%。更多还原

【Abstract】 The optimum extraction conditions of Tannin from Galla Chinensis were studied by using orthogonal test L₁₆（4⁴）.Then separation and purification of Tannin were studied by ethyl acetate extraction,gelatine precipitation and Ca²⁺precipitation.The optimum extraction process conditions were as follows:100% acetone as extracting agent,extracting temperature 50℃,pH value 2.0,required time 10 min.The results show that acetone as extraction agent and the ethyl acetate extraction,the purification degree was up to 89.15%.The yield was also relatively high ethyl acetate extraction.更多还原