【作者】 赵亮； 陈忠正； 文海涛； 李斌； 郭健；

【Author】 Zhao Liang Chen Zhongzheng Wen Haitao Li Bin Guo Jjian (College of Food Science,South China Agricultural University,Guangzhou 510642,China)

【机构】 华南农业大学食品学院；

【摘要】 cDNA 文库的构建是对基因进行表达及研究的重要手段之一,随着基因工程技术的迅猛发展,cDNA 文库的构建方法也得到了巨大的改进和提高,多种全长文库的构建方法以及多种类型文库的构建策略大大方便了功能基因的筛选及克隆,本文就试对 cDNA 文库的构建方法及策略做一简要综述。更多还原

【Abstract】 Construction of cDNA library is an important way to research the genes and its expressions.As to the rapid development of genetic engineering,methods of cDNA library construction has been improved greatly,many different ways of full-length cDNA construction and different types of construction strategy tremendously convenience the clone of gene and its screen.This article summarize the methods of cDNA library construction and its strategy recently.更多还原