【作者】 于平； 励建荣；

【Author】 Yu Ping Li Jianrong (Department of Bioengineering,Zhejiang Gongshang University,Hangzhou 310035)

【机构】 浙江工商大学生物工程系；

【摘要】 为研究从螺旋藻中分离纯化 SOD 的最优化方法,采用细胞破碎方法、最佳丙酮用量、最佳硫酸铵饱和度和 DEAE-52离子交换层析技术分离纯化螺旋藻中的 SOD 并作比较分析。结果表明：从螺旋藻中分离纯化的 SOD 的比活高达2179 U/mL,提纯倍数24．2；纯化的 SOD 经 SDS-PAGE 分析,呈现的谱带均一且对 H2O2非常敏感,推断为 Fe-SOD。因此采用优化技术可以从螺旋藻中分离纯化获得高纯度和高比活的 SOD。更多还原

【Abstract】 In order to study the optimal methods for isolate and purify SOD from Spirulina platensis,cell-broken methods,the optimal amount of acetone,the optimal saturation of ammonium sulfate and the conditions of DEAE-52 iron exchange chromatography were adopted and compared.Results showed that the specific activity and the purified fold of SOD obtained attained to 2 179 U/mL and 24.2,respectively.The uniform band of SOD was presented on SDS-PAGE electrophoretical map.SOD obtained was sensitive to H2O2,so it is Fe-SOD.The SOD with high specific activity and high purity may obtain from Spirulina platensis by this method.更多还原