【作者】 张丽萍； 张东杰； 冯昆；

【Author】 Zhang Liping Zhang Dongjie Feng Kun (Heilongjiang August First Agricultural Reclamation University Food college,Daqing 163319)

【机构】 黑龙江八一农垦大学食品学院；

【摘要】 目的：研究苜蓿初步提纯组分的得率及纯化组分对 HIV 逆转录酶和蛋白酶活性的体外抑制效果。方法：使用热水浸提法提取芷蓿粗多糖,经 DEAE-纤维素柱分段洗脱,得 S1、S2、S3、 S4 4种组分；采用试剂盒对体外 HIV 逆转录酶和蛋白酶活性的抑制作用进行检测比较。结果：S3 和 S4对 HIV 逆转录酶活性的抑制率较高,分别为99．6%和76．7%；S1、S2对 HIV 蛋白酶活性的抑制率较高,分别为58．6%和55．9%。结论：S3和 S4表现出对 HIV 逆转录酶活性有很强的抑制作用, S1、S2对 HIV 蛋白酶活性有较强的抑制作用。更多还原

【Abstract】 Objective:To research the rate gained from the refined components,to research the inhibited result of the refined components in vitro.Methods:The coarse medicago sativa polysaccharides was soaked with hot water and flushed away in subsection with DEAE-Cellulose column.As a result,four components S1、S2、S3 and $4 were gained;Then,the activities of the reverse transcriptase of HIV and the protease of HIV were detected and compared through the test kit.Result: S3 and S4 were higher inhibition rate to the reverse transcriptase of HIV,99.6% and 76.7% respectively;S1 and S2 were higher of the protease of HIV,58.6% and 55.9% respectively. Conclusion:S3 and S4 showed a higher inhibiting function to the activity of the reverse transcriptase of HIV.in contrast,S1 and S2 showed a higher inhibiting function to the activity of the protease of HIV.更多还原