【作者】 艾启俊； 于庆华； 吴小虎； 张伟；

【Author】 AI Qi-jun1 YU Qing-hua1,2 WU Xiao-hu1 ZHANG Wei1 ~1Department of Food Science, Beijing University of Agriculture, Beijing 102206;2 College of Food Science and Food Science and Engineering Department,Inner Mongolia Agriculture University,Huhhot 010018,China

【机构】 北京农学院食品系；

【摘要】 对鹿蹄草素作用下金黄色葡萄球菌和绿脓杆菌进行扫描电镜观察,从形态学角度初步探讨了鹿蹄草素抑菌和杀菌机理。结果表明：鹿蹄草素作用后金黄色葡萄球菌菌体细胞结构被明显破坏,细胞壁呈溶解状,菌体细胞间相互粘结,细胞间界限变模糊；绿脓杆菌菌体细胞间界限变模糊,但并未对菌体细胞壁和细胞膜造成破坏。结果分析认为,鹿蹄草素对金黄色葡萄球菌的抑制和杀灭与菌体细胞膜和细胞壁结构被破坏直接相关,而鹿蹄草素对绿脓杆菌的抑制与菌体细胞膜和细胞壁无关。更多还原

【Abstract】 In order to initially investigate antimicrobial mechanisms of pyrolin against Staphylococcus S.aureus and Pseudomonas aeruginosa,the structure under scanning electron microscope was studied.The results show that,Pyrolin bad strong bactericidal action against S.aureus.The membrance patency of the cells affected by pyrolin of S.aureus compared with that of the normal cells dramatically increased.But that of P.aeruginosa did not change.Observation under scanning electron microscope revealed that the structure of the cells of S.aureus were significantly destroyed by pyrolin,and the cells wall were melted.The cells were sticked by the melt,the boundary between cells became obscure.The boundary between cells of P.aeruginosa also got obscure,but the structure of it was not destroyed by pyrolin.In conclusion,the antimicrobial activity of Pyrolin against S.aureus is relevant to the structure destruction of cells membrance and wall.But that of P.aeruginosa is not.更多还原