【作者】 张伟； 艾启俊； 吴小虎；

【Author】 Zhang Wei~1 Ai Qijun~2 Wu Xiaohu~1 (1.Department of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China) (2.Department of Food Stuff Science,Beijing University of Agriculture,Beijing 102206,China)

【机构】 北京农学院植物科学技术系； 北京农学院食品科学系；

【摘要】 为寻找天然果蔬防腐剂,本试验在离体条件下,采用生长速率法和培养板连续稀释法研究了鹿蹄草素对几种果蔬采后病原真菌的抑制效应,并对其最小抑菌浓度(MIC)和最小杀菌浓度 (MBC)进行了初步确定。结果表明：鹿蹄草素的浓度越高,抑菌率越高,在2．5mg/mL 及以上浓度时对供试病原真菌均有较好的抑制效果。根据 EC50值所得到的抑制作用由强到弱依次为链核盘菌(Monilinia fructicola),苹果轮纹病菌(Physalospora piricola),链格孢菌(Alternaria alternata), 根霉菌(Rhizopus stolonifer),灰葡萄孢菌(Botrytis cinerea)。其中链核盘菌的 MIC 和 MBC 分别为： 0．156,1．25mg/mL；苹果轮纹病菌的 MIC 和 MBC 分别为：0．078,0．156 mg/mL；链格孢菌的 MIC 和 MBC 分别为：0．313,0．625mg/mL；灰葡萄孢菌的 MIC 和 MBC 分别为：0．156,0．313 mg/mL．更多还原

【Abstract】 Agar plate tests and dilution methods were conducted to test the inhibitory effects in-vitro of pyrolin in order to search the new type natural antisepic agent.And its MIC and MBC were preliminarily identified.The results showed that higher the concentration of Pyrolin,higher the antimicrobial effects. Pyrolin had anti-microbial activity on all the pathogenic fungi tested when concentrations of pyrolin was 2.5 mg/mL and above.Inhibitory effects were weakened with an order of Monilinia fructicola,Altemaria alternate,Physalospora piricola,Botrytis cinerea,Rhizopus stolonifer according to the EC50.The MIC and MBC of Monilinia fructicola were 0.156 mg/mL and 1.25 mg/mL respectively,The MR2 and MBC of Alternaria alternata were 0.313 mg/mL and 0.625 mg/mL respectively;The MIC and MBC of Physalospora piricola were 0.078 mg/mL and 0.156 mg/mL respectively;The MIC and MBC of Botrytis cinerea were 0.156 mg/mL and 0.313 mg/mL respectively.更多还原