17β-EstradioI and raloxifene acutely reduce glutamate-induced intracellular calcium increase in primarily cultured neurons through a membrane receptor-dependent process

【作者】 黄莺； 黄娅林； 张笋； 朱依纯； 姚泰；

【机构】 复旦大学上海医学院生理与病理生理学系；

【摘要】 <正>Increasing lines of evidence indicate that estrogen acts as a neuroprotective agent through a non-genomic mechanism. The current study investigated whether 17β-estradiol and the selective estrogen receptor modulator raloxifene could rapidly attenuate glutamate-induced calcium (Ca2+) overload in rat primary neurons via a membrane receptor-dependent process. The bulk cytosolic intracellular Ca2+ level was measured in neurons with fluorescent Ca2+ probe fluo3. Pre-exposure of primary cultured更多还原