【作者】 薛艳； 周东美； 郝秀珍； 沈振国； 陈怀满；

【Author】 XUE Yan1,2, ZHOU Dong-mei1, HAO Xiu-zhen1, SHEN Zhen-guo2, CHEN Huai-man1 (1. State Key laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008;2. College of Life Science, Nanjing Agricultural University, Nanjing 210095)

【机构】 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室； 南京农业大学生命科学院；

【摘要】 选择了具有耐性差异的两个青菜品种火青菜和四月慢为供试材料,通过水培试验考察了它们对铜的吸收、根系活力、叶绿素、氨基酸和谷胱甘肽(GSH)含量以及铜结合蛋白等生理生化指标的影响。结果表明:四月慢较火青菜对铜有较快的吸收;与四月慢相比,火青菜在铜的胁迫条件下能够产生更多的氨基酸和GSH,并表现出更强的根系活力。根系蛋白凝胶色谱结果显示有两个峰出现,分别为大分子的铜结合蛋白和小分子的Cu-PCs。不同青菜品种对铜耐性的差异与它们的生理生化响应过程有关。更多还原

【Abstract】 Response mechanisms of two cultivars of B. chinensis to Cu were investigated using hydroponic culture experiments. The results showed that the root Cu concentration was higher than that of the shoot in both the two cultivars of Brassica chinensis. With the increasing cultivation period, the Cu concentration in root and shoot in the same cultivar had the same accumulation tendency, but it is different between the different cultivars. In comparison with the cultivar Siyueman, Cu treatment more strongly increased the content of amino acids, GSH, and the root livingness as well for the cultivar Huoqingcai. There are two Cu peaks appeared in the dilution diagram of plant root by Sephadex G-50.更多还原