【作者】 金雅磊； 王捷荣； 王红玲； 张渝侯；

【Author】 JIN Ya-lei WANG Jie-rong WANG Hong-ling et al(Department of Pediatrics.The second hospital,Hu Bei Medical University,Wu Hart,430071)

【机构】 湖北医科大学附属第二医院；

【摘要】 目的:比较γIL-2及抗 CD3mcAb+γIL-2对脐血单个核细胞(MNC)的免疫表型及细胞因子的影响。方法:采用间接玫瑰花结法实验测定23例脐血 MNC 表面的分化抗原,ELISA 双抗夹心法测定肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、白介素-8(IL- 8)。结果:经γIL-2及抗 CD3mcAb+γIL-2激活的脐血 MNC 其分化抗原发生明显变化。1、两组与未活化相比:CD3~+、CD8~+、CD16~+、 CD56~+、CD19~+、CD23~+、HLA-DR ↑,其表达 CD25亦均增加,但抗 CD3mcAb+γIL-2激活组>γIL-2组(P<0.01),CIMSRA+均明显下降, 但两组差异无显著性(P>0.05),CD34~+无明显改变。2、经抗 CD3mcAb+γIL-2激活的 MNC,细胞集落增加,但γIL-2组无此作用。3、细胞因子的改变,无论经γIL-2还是抗 CD3mcAb+γIL-2激活的脐血 MNC 上清液,其 IL-6增高,但差异无显著性(P>0.05),TNF-α,IL-8亦明显增高,但抗 CD3mcAb 组>γIL-2组(P<0.01)。结论:脐血抗 CD3激活的杀伤细胞(CD3AK)比γIL-2激活的 LAK 毒性作用更强,更有利于杀伤肿瘤细胞。更多还原

【Abstract】 Objective:To compare and analyse the antigen expression of monocyte and cytokine of human umbilical cord blood activated byγIL-2 andγIL-2+Anti-CD3mcAb.Methods:By using indirect resette forming test,antigen expression rate of mono- cyte in 23 cases was detected.The levels of interleukin-8(IL-8),intefleukin-6(IL-6)and tumor necrosis factorct(TNF-α)of 23 cases were measured by ELISA.Results:After activated by Anti-CD3mcAb and/orγIL-2,The antigen expression of monocyte and cytekine are obviously changed:(1)inγIL-2 group andγIL-2+Anti-CD3mc Ab group,CD3~+ ,CD8~+,CD56~+,HLA-DR~+, CD19~+ ,CD25~+↑,butγIL-2+Anti-CD3mcAb group>γIL-2 group(P<0.01),CD45RA+↑,But there are no difference be- tween two groups(P>0.05).CD34+cells(also named hemopoietic stem cell)are no significant change.(2)The number of celluar colony is increased in Anti-CD3mcAb+γIL-2 group,but it is no change inγIL-2 group.(3)The change of cytokine:Both of the levels of IL-8,TNF-αare increased in the sreum of cord blood in two groups,ButγIL-2+Anti-CD3mcAb group>γIL-2 group (P<0.01).The levels of IL-6 in two groups is also higher thanunactivated group.Conclusions:Anti-CD3mcAb activated killer ceils are stronger cytoxicity than lymphikine-activated killer cells,therefore it maybe have advantage of killing tumor.更多还原