【作者】 谢嵘； 朱剑虹；

【Author】 Xie Rong Zhu Jian - Hong Departments of Neurosurgery, Fudan University Huashan Hospital, National Key Laboratory for Medical Neurobiology, Shanghai Medical College - Fudan University, Shanghai, China 200040

【机构】 复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心；

【摘要】 <正>Obejctive: To establish detailed protein expression maps of central nervous system bemangioblastomas (CNS HB) and identify discrepant proteins between HB and normal brain tissues by using proteomics analysis so as to better understand the histological origin of CNS HB.Methods: Ten individual brain regions with five HB tissues (HB group) and five normal cerebellar tissues (control group) were included.Total proteins were extracted from the samples and process of two dimensional gel electrophoresis.spot excision,in- gel digestion, MALDI - TOF MS and bioinformatics analysis were carried out. Results: After 2 - DE, high - resolution maps containing about 600 protein spots of HB and normal brain tissues were produced.The 2 - DE maps show a satisfactory homology between HB and normal brain tissues. By using the ImageMaster 2D software, 115 discrepant protein spots between HB and normal brain tissues could be selected and 87 of them were successfully identified by MALDI - TOF MS. Among them,46 proteins expressions were up - regulated while 41 were down - regulated. According to their functional role,the identified proteins could be divided into several groups including transport,protein metabolism and folding,glycolysis and stem cell proteins,et al.The results of immunohistochemistry staining of EPO, Vimentin and 14-3-3 proteins could authenticate the validity of the proteomics analysis in this study. Conclusions: The process of CNS HB occurring is an intricate, multicomponent, multifactorial and multi-step process which is mediated by a variety of proteins.CNS HB as a kind of tumors may come from normal brain mesenchymal tissues.Several proteins such as EPO、Vimentin and 14-3-3 may play an important role in the occurring of HB.更多还原

【Abstract】 Obejctive: To establish detailed protein expression maps of central nervous system bemangioblastomas (CNS HB) and identify discrepant proteins between HB and normal brain tissues by using proteomics analysis so as to better understand the histological origin of CNS HB.Methods: Ten individual brain regions with five HB tissues (HB group) and five normal cerebellar tissues (control group) were included.Total proteins were extracted from the samples and process of two dimensional gel electrophoresis.spot excision,in- gel digestion, MALDI - TOF MS and bioinformatics analysis were carried out. Results: After 2 - DE, high - resolution maps containing about 600 protein spots of HB and normal brain tissues were produced.The 2 - DE maps show a satisfactory homology between HB and normal brain tissues. By using the ImageMaster 2D software, 115 discrepant protein spots between HB and normal brain tissues could be selected and 87 of them were successfully identified by MALDI - TOF MS. Among them,46 proteins expressions were up - regulated while 41 were down - regulated. According to their functional role,the identified proteins could be divided into several groups including transport,protein metabolism and folding,glycolysis and stem cell proteins,et al.The results of immunohistochemistry staining of EPO, Vimentin and 14-3-3 proteins could authenticate the validity of the proteomics analysis in this study. Conclusions: The process of CNS HB occurring is an intricate, multicomponent, multifactorial and multi-step process which is mediated by a variety of proteins.CNS HB as a kind of tumors may come from normal brain mesenchymal tissues.Several proteins such as EPO、Vimentin and 14-3-3 may play an important role in the occurring of HB.更多还原