【作者】 朱剑虹； 谢嵘； 陈露萍； 沈亦雯； 毛颖； 周良辅；

【Author】 Zhu Jian-Hong Xie Rong Chen Lu-Ping Shen Yi-wen Mao Ying Zhou Liang-Fu Departments of Neurosurgery, Fudan University Huashan Hospital, National Key Laboratory for Medical Neurobiology, Shanghai Medical College-Fudan University, Shanghai, China 200040

【机构】 复旦大学附属华山医院神经外科复旦大学上海医学院医学神经生物学国家重点实验室上海市神经外科临床医学中心；

【摘要】 <正>Objective: To better understand the cell origin and pathogenetic step of central nervous system he-mangioblastomas (CNS HB). Methods: 14 VHL-associ-ated CNS HB, 21 none VHL-associated CNS HB and 15 normal brain tissues were collected and all the specimens were sectioned and subjected to routine histology and im-munohistochemistry analysis. Avidin-biotin-complex immunoperoxidase was used to evaluate the expression of CD31, CD34, CD117, CD133, Nestin, erythropoietin (EPO) in CNS HB. Another three mixed samples collected from 5 VHL-associated CNS HB, 7 none VHL-asociated CNS HB and 7 normal brain tissues were analyzed by using Oligo cDNA microarray to screen the stem cells markers, of which the gene expression levels had distinct differences among VHL associated HB, none VHL associated HB and normal brain tissues. RT-PCR and Western blot was used to confirm the validity of the results. Results: After inunu-nochemistry analysis, all CNS HB pathological sections were observed to express stem cells markers including CD31, CD34, CD117, CD133, Nestin. The results of cDNA microarray showed higher gene expression levels of several important stem cells markers including ABCG2, AXIN1, BMP2, CD3, CD4, etc of CNS HB than those of normal brain tissues and no expression difference of the markers was found between VHL and none VHL-associated HB. Both immunochemistry and cDNA microarray suggested that EPO was highly expressed in CNS HB but the expression level of VHL-associated HB was higher than that of none VHL-associated HB. Conclusions: The study suggests that CNS HB can express markers of mes- enchymal stem cells and may have an origin of mesenchy-mal stem cells. The expression of EPO may be a critical pathogenetic step as a cell proliferation stimulus but has different contributions in VHL and none VHL-associated HB.更多还原

【Abstract】 Objective: To better understand the cell origin and pathogenetic step of central nervous system he-mangioblastomas (CNS HB). Methods: 14 VHL-associ-ated CNS HB, 21 none VHL-associated CNS HB and 15 normal brain tissues were collected and all the specimens were sectioned and subjected to routine histology and im-munohistochemistry analysis. Avidin-biotin-complex immunoperoxidase was used to evaluate the expression of CD31, CD34, CD117, CD133, Nestin, erythropoietin (EPO) in CNS HB. Another three mixed samples collected from 5 VHL-associated CNS HB, 7 none VHL-asociated CNS HB and 7 normal brain tissues were analyzed by using Oligo cDNA microarray to screen the stem cells markers, of which the gene expression levels had distinct differences among VHL associated HB, none VHL associated HB and normal brain tissues. RT-PCR and Western blot was used to confirm the validity of the results. Results: After inunu-nochemistry analysis, all CNS HB pathological sections were observed to express stem cells markers including CD31, CD34, CD117, CD133, Nestin. The results of cDNA microarray showed higher gene expression levels of several important stem cells markers including ABCG2, AXIN1, BMP2, CD3, CD4, etc of CNS HB than those of normal brain tissues and no expression difference of the markers was found between VHL and none VHL-associated HB. Both immunochemistry and cDNA microarray suggested that EPO was highly expressed in CNS HB but the expression level of VHL-associated HB was higher than that of none VHL-associated HB. Conclusions: The study suggests that CNS HB can express markers of mes- enchymal stem cells and may have an origin of mesenchy-mal stem cells. The expression of EPO may be a critical pathogenetic step as a cell proliferation stimulus but has different contributions in VHL and none VHL-associated HB.更多还原