【作者】 王世宏； 吴强； 肖志坚；

【Author】 Wang Shi-Hong Wu Qiang Xiao Zhi-Jian State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, CAMS & PUMC,Tianjin 300020,China; FT-IR Laboratory, High Polymer Material Speciality, Department of Chemistry, Nankai University) Shihong Wang

【机构】 中国医学科学院中国协和医科大学血液学研究所血液病医院实验血液学国家重点实验室血液内科；

【摘要】 目的:本研究旨在应用傅立叶转换红外光谱(Fourier Transform Infrared,FT—IR)探讨骨髓增生异常综合症 (MDS)、急性髓系白血病M2a(AML-M2a)患者DNA结构的异常机制,寻找可能的MDS和AML-M2a临床早期诊断和预测方法。方法:通过对上述病人和正常入骨髓标本分离单个核细胞(MNC)并提取DNA,将DNA彻底干燥,然后对干燥的DNA样本进行FT—IR光谱扫描分析,对初治和复治 MDS、急性髓系白血病M2a病人和正常对照组单个核细胞 DNA结构进行红外光谱分析研究。结果:各组均有一致的光谱规律;MDS组与正常组相比,谱图(吸光度)明显不同, 找出了其异常的谱图规律,急性髓细胞性白血病M2组与正常组相比,结果显著不同,找出了其异常的谱图规律, 急性非淋巴细胞性白血病与MDS组比较,谱图存在一定差异,找到了其不同的规律。结论:骨髓增生异常综合征和急性髓系白血病M2a的基因组DNA磷酸核糖主链、磷酸骨架和碱基结构存在明显异常,可能为MDS发生发展中的初始变故,傅立叶转换红外光谱分析是一种MDS和AML-M2a有潜在应用价值的早期诊断和预测方法。更多还原

【Abstract】 Objective: The study aim to explore the mechanism of abnomal DNA structure in acute myeloid leukemia(AML) and myelodysplastic syndrome(MDS) by Fourier Transform Infrared(FT-IR)spectroscopy and to explore the methods of early diagnosis and prognosis. Methods To segregate the mononuclear cell of AML and MDS,extract the DNA, and then check the dry DNA by FT-IR spectroscopy. Analyse the identical and different among AML, MDS and Normal groups. Results: Each group has a almost concordant FTIR spectroscopy rule. Compared with the control group, AML and MDS FTIR were significant diference. And also signiflcant diference are between AML and MDS. We have found the rules. Conclusion: The principal chain, The phosphonic backbone, and phosphonic basyl of DNA in AML and MDS have significant diference with the Normal control and could be the initial change in AML and MDS occurrence and development.To AML and MDS, FTIR spectroscopy is a potential method of early diagnosis and prognosis.更多还原