【作者】 卿三华； 李镠洋； 盛新华； 巴明臣；

【Author】 Qin San-Hua Li Liu-Yang Sheng Xin-Hua Department of General Surgery, Nanfang Hospital, Nanfang medical University, Guangzhou 510515, China.

【机构】 南方医科大学南方医院普通外科；

【摘要】 目的:应用profrinII纳米微粒PDT对结肠癌进行体外实验研究,探讨纳米化profrinII-PDT作为一种新型疗法治疗结肠癌的可行性。方法:1、建立人结肠癌LoVo细胞裸鼠种植瘤模型,2、将光敏剂profrinII纳米化并观察其理化性质,3、经荷瘤鼠尾静脉注入profrinII纳米微粒(30m g／ k g体重),光敏化3 h后半导体激光仪垂直照射肿瘤30m i n(能量密度9 J／c m 2),照射后连续观察肿瘤体积,瘤体 H E染色病理分析,观察纳米化profrinII—PDT对结肠肿瘤的杀伤抑制作用及组织形态学影响。结果:1、profrinII纳米化后粒子多呈球型,分布比较均匀,平均粒经为62nm,2、 profrinII纳米微粒P D T在治疗后早期产生明显的抑制肿瘤增殖作用,体积抑瘤率达72．8％(P<0．05),而于治疗后期, 肿瘤体积仍会缓慢增长,但增长速度明显均小于对照组,对照组对肿瘤生长增殖没有明显影响,PDT治疗后早期(7d)可引起瘤体组织坏死,腺腔样结构解离破坏,而在治疗后期 (21d)可观察到坏死灶与肿瘤细胞团并存,3、与对照组相比, 纳米化profrinII—PDT可明显延长荷瘤裸鼠的生存期,达 38．0±6．0天,延命率为65．2％。结论:1、应用纳米化 profrinII—PDT治疗后可明显抑制裸鼠种植瘤的生长,延长荷瘤裸鼠的生存期,虽然随着时间延长,肿瘤体积仍会缓慢增长．但增长速度明显小于对照组,2、与普通DPT相比profrinII 纳米微粒P D T抑瘤率亦达28．8％,普通profrinII—PDT治疗的平均生存时间为(32．0±4．0)d,profrinII纳米微粒PDT 治疗后平均生存期达(38．0±6．0)d,延命率为18．2％,利用纳米技术改良光敏剂可提高直肠癌光动力治疗效果。更多还原

【Abstract】 Objective: To evaluate the inhibitory of profrinll nanopartides photodynamic therapy on LoVo human colon cancer xenografts in athymic mice. Methods: Profrinll nanopartides were obtained from hypersound emulsification method. LoVo human colon cancer xenograft were established in athymic mic. Athymic mic were divided into four groups: normal control group, profrinll nanopartides control group, profrinll PDT group and profrinll nanopartides PDT group. The animals bearing xenografts were treated 30mg/kg body weight profrinll nanopartides and 3 h later were irradiated with 9J/cm2 light from a diode laser. After Profrinll nanoparticles PDT, the anti-tumor effect was assessed by messuring tumor valume over a 3-4weeks period, the morphologic changers were observed by macroscopy and microscopy via the histological examination. Results: Compared with the control groups and profrinll PDT group, profrinll nanoparticles-PDT-treated tumor had regressed significantly in earlier period with the inhibititing rate being 72.8%(P <0.05) and 28.2%( P<0.05). In the later period post-PDT, tumors growth resumed with a slower rate. Profrinll nanoparticles-PDT polonged the survival time in the treated group with (38.0 ± 6.0 ) days (P <0.05), Extensive damage to tumor tissue was found in the earlier period ( 7d ) post-PDT, whereas in the later period (21d) post-PDT, islands of vital-looking tumor cells were observed around the damage tissue. Conclusion: Profrinll nanoparticles-PDT results in inhibition LoVo colon carcinoma growth in post-PDT earlier period in vivo, and can prolong the survival time of nude mice bearing xenografts significantly, whereas it seems that profrinII-PDT could not inhibite the growth of colon tumor completely.更多还原