【作者】 赵勇； 高建飞； 欧武陵； 朱宇泽； 章必成； 杜光祖；

【Author】 Zhao Yong Ou Wu-Ling Zhang Bi-Cheng et al. Department of Oncology Wuhan General Hospital,Guangzhou Military Command, PLA, Wuhan 430070, China

【机构】 广州军区武汉总医院肿瘤科；

【摘要】 目的:探讨大肠癌hMLH1蛋白表达与5-Fu敏感性的关系。方法:应用流式细胞术测定大肠癌hMLH1蛋白表达,用体外肿瘤药物敏感实验(MTT法)检测大肠癌细胞对5-Fu敏感性,并分析hMLH1蛋白表达与大肠癌细胞对5- Fu敏感性的关系。结果:大肠癌hMLH1蛋白阳性和阴性之间5-Fu敏感性存在显著性差异(x2=5．97,P<0．05)。结论: 临床标本中hMLH1蛋白表达缺失与大肠癌细胞对5-Fu耐药密切相关。更多还原

【Abstract】 Objective: To investigate the correlation of hMLH1 protein expression and sensitivity of 5-fluo-rouracil in colorectal cancer. Methods: Flow cytometry was used to detect the expression of hMLH1 in colorectal caner and the sensitivity of 5-fluorouracil was measured by MTT test method. Results: The hMLH1 positive cancer was more sensitive to 5-fluorouracil than hMLH1 negative tumor ( x2=5.97, P<0.05 ).Conclusion: A lack of hMLH1 expression may play a role in 5-fluorouracil resistance in colorectal cancer.更多还原