【作者】 刘剑勇； 李文锋； 张志明； 赵荫农； 崔英； 吕丽琼； 罗善超； 张力图； 张春燕；

【Author】 Liu Jian-Yong Li Wen-Feng Zhang Zhi-Ming et al Affiliated Cancer Hospital of Guangxi Medical University, Nanning 530021, China

【机构】 广西医科大学附属肿瘤医院肝胆乳腺外科； 广西医科大学附属肿瘤医院实验研究部；

【摘要】 目的:旨在探讨小鼠H22肝癌细胞全细胞性抗原致敏的DC(树突状细胞)联合SEA(葡萄球菌肠毒素A)激活的 TIL(肿瘤浸润淋巴细胞)体外抗小鼠肝癌活性作用。方法:从荷瘤小鼠四肢长骨骨髓中获取DC,应用粒／巨噬细胞集落刺激因子(GM—CSF)、白介素-4(IL-4)和小鼠H22肝癌细胞全细胞性抗原致敏DC,然后用已致敏的DC联合SEA激活从荷瘤小鼠中提取的TIL和脾淋巴细胞,观察受激活后的TIL 和脾淋巴细胞作为效应细胞在体外对小鼠H22肝癌细胞和小鼠S180内瘤细胞的杀伤活性。并与对照组进行比较。结果: 各组效应细胞对小鼠H22肝癌细胞的杀伤活性均高于各自对应的效应细胞对小鼠S180肉瘤细胞的杀伤活性,TIL对小鼠 H22肝癌细胞的杀伤活性[杀伤率为(50．63+2．52)％]高于脾淋巴细胞对小鼠H22肝癌细胞的杀伤活性[杀伤率为 (12．26+1．28)％],用不同方式激活的TIL对小鼠H22肝癌细胞的杀伤活性均高于各自对应方式激活的脾淋巴细胞对小鼠 H22肝癌细胞的杀伤活性,SEA激活的TIL对小鼠H22肝癌细胞的杀伤活性[杀伤率为(64．35+2．82)％]高于TIL对小鼠 H22肝癌细胞的杀伤活性[杀伤率为(50．63+2．52)％],已致敏 DC激活的TIL对小鼠H22肝癌细胞的杀伤活性更高[杀伤率为(75．23+3．21)％],而已致敏DC联合SEA激活的TIL对小鼠H22肝癌细胞的杀伤活性最高[杀伤率为(86．29+3．83)％]。结论:用小鼠H22肝癌细胞全细胞性抗原致敏的DC联合 SEA能显著激活TIL产生高效而特异的对小鼠H22肝癌细胞的杀伤活性。更多还原

【Abstract】 Objective: To investigate the killing activity of TIL that were stimulated by S-DC (DC had been sensitized by H22 cell antigen) and SEA on mouse hepa-tocellular carcinoma vitro. Methods: DC were isolated from mouse bone marrow and sensitized by granulo-cyte/ macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4) and H22 cell antigen. Then TIL and mouse spleen Iymphocytes(the cells were from mouse bearing tumor )were stimulated by S-DC and SEA, and compared their killing activity with the controls on H22 and S180 cells in vitro. Results: The killing rate of every effector cell group on H22 cells was higher than that of corresponding control group on S180 cells respectively. The killing activity of TIL on H22 cells [killing rate: (50.63+2.52)%] was higher than that of spleen lymphocytes on H22 cells [killing rate: (7.26+1.28) %].The killing rate of TIL that were stimulated by difference manners on H22 cells was higher than that of spleen lymphocytes that were stimulated by corresponding difference manners on H22 cells respectively. TIL were stimulated by SEA had higher killing activity[killing rate: (64.35+ 2.82)%] than that of TIL on H22 cells[killing rate: (50.63+2.52) %],TIL were stimulated by S-DC had much higher killing activity on H22 cells [killing rate: (75.23+3.21)%],TIL were stimulated by S-DC and SEA had the highest killing activity [killing rate: (86.29+ 3.83) %]in all groups. Conclusion: S-DC and SEA can induce the most efficient and specific killing activity of TIL on H22 cells in vitro.更多还原